Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, Singapore, Singapore.
Methods Mol Biol. 2024;2707:99-110. doi: 10.1007/978-1-0716-3401-1_6.
Small teleost fishes such as zebrafish and medaka show remarkable regeneration capabilities upon tissue injury or amputation. To elucidate cellular mechanisms of teleost tissue repair and regeneration processes, the Cre/LoxP recombination system for cell lineage tracing is a widely used technique. In this chapter, we describe protocols used for inducible Cre/LoxP recombination-mediated lineage tracing of osteoblast progenitors during medaka fin regeneration as well as during the repair of osteoporosis-like bone lesions in the medaka vertebral column. Our approach can be adapted for lineage tracing of other cell populations in the regenerating teleost fin or in other tissues undergoing repair.
小型硬骨鱼类，如斑马鱼和青鳉，在组织损伤或截除后表现出显著的再生能力。为了阐明硬骨鱼组织修复和再生过程中的细胞机制，Cre/LoxP 重组系统用于细胞谱系示踪是一种广泛使用的技术。在本章中，我们描述了用于诱导 Cre/LoxP 重组介导的青鳉鳍再生过程中以及骨质疏松样骨病变修复过程中骨祖细胞谱系示踪的方案。我们的方法可以适用于再生硬骨鱼鳍或其他正在修复的组织中其他细胞群体的谱系示踪。
