Advanced Bioprocessing Centre, sBrunel Institute for Bioengineering, Brunel University, Kingston Lane, Uxbridge, Middlesex UB8 3PH, UK.
J Chromatogr A. 2011 Aug 12;1218(32):5527-30. doi: 10.1016/j.chroma.2011.04.013. Epub 2011 Apr 14.
Two different laboratory scale liquid-liquid extraction processes using aqueous two-phase systems (ATPS) are compared: centrifugal partition chromatography (CPC) and multilayer toroidal coil chromatography (MTCC). Both use the same phase system, 12.5% (w/w) PEG-1000:12.5% (w/w) K(2)HPO(4), the same flow rate of 10 mL/min and a similar mean acceleration field of between 220 × g and 240 × g. The main performance difference between the two processes is that there is a continuous loss of stationary phase with CPC, while for MTCC there is not - even when sample loading is increased. Comparable separation efficiency is demonstrated using a mixture of lysozyme and myoglobin. A throughput of 0.14 g/h is possible with CPC despite having to refill the system with stationary phase before each injection. A higher throughput of 0.67 g/h is demonstrated with MTCC mainly due to its ability to tolerate serial sample injections which significantly reduces its cycle time. While CPC has already demonstrated that it can be scaled to pilot scale, MTCC has still to achieve this goal.
两种不同的实验室规模液-液萃取工艺（ATPS）使用两相系统（ATPS）进行比较：离心分配色谱（CPC）和多层环形线圈色谱（MTCC）。两者均使用相同的相系统，即 12.5%（w/w）PEG-1000：12.5%（w/w）K（2）HPO（4），相同的流速为 10 mL/min，平均加速度场在 220×g 和 240×g 之间。两种工艺之间的主要性能差异在于 CPC 存在连续损失固定相的问题，而 MTCC 则没有，即使增加了样品负载。使用溶菌酶和肌红蛋白的混合物证明了类似的分离效率。尽管 CPC 在每次进样前都必须用固定相重新填充系统，但仍可以达到 0.14 g/h 的吞吐量。MTCC 的吞吐量高达 0.67 g/h，主要是因为它能够耐受连续的样品进样，这大大缩短了其循环时间。尽管 CPC 已经证明它可以扩展到中试规模，但 MTCC 仍需要实现这一目标。
