School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba, 274-8555, Japan.
College of Science and Technology, Nihon University, 7-24-1 Narashinodai, Funabashi, Chiba, 274-8501, Japan.
Anal Sci. 2021 Feb 10;37(2):359-365. doi: 10.2116/analsci.20P292. Epub 2020 Oct 23.
The partition efficiency of the double-spaced coil for eccentric and toroidal coils on countercurrent chromatographic separation of proteins was evaluated using the small-scale cross-axis coil planet centrifuge (CPC) equipped with circular and elliptic cylindrical columns. Standard cytochrome c, myoglobin and lysozyme samples were used for separation with the 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate system. In the circular column, the double-spaced eccentric coil yielded better peak resolution than the double-spaced toroidal coil, and the double-spaced eccentric coil yielded better peak resolution than the single-spaced eccentric coil. In the elliptic column, the double-spaced eccentric coil also produced better peak resolution than the double-spaced toroidal coil, but the single-spaced eccentric coil yielded better peak resolution than the double-spaced eccentric coil. The overall results indicated that the double-spaced eccentric coil for the circular column and the single-spaced eccentric coil for the elliptic column yielded better protein separation using the small-scale cross-axis CPC with aqueous two-phase solvent systems.
采用配备有圆形和椭圆形柱的小型横轴盘管行星离心机（CPC），评估了双间隔螺旋线圈在逆流色谱蛋白质分离中对偏心和环形线圈的分配效率。使用标准细胞色素 c、肌红蛋白和溶菌酶样品，在 12.5%（w/w）聚乙二醇 1000 和 12.5%（w/w）磷酸氢二钾系统中进行分离。在圆形柱中，双间隔偏心线圈的峰分辨率优于双间隔环形线圈，而双间隔偏心线圈的峰分辨率优于单间隔偏心线圈。在椭圆形柱中，双间隔偏心线圈的峰分辨率也优于双间隔环形线圈，但单间隔偏心线圈的峰分辨率优于双间隔偏心线圈。总体结果表明，使用配备有水相两相溶剂系统的小型横轴 CPC，圆形柱的双间隔偏心线圈和椭圆形柱的单间隔偏心线圈可实现更好的蛋白质分离。
