Mann D M, Brown A M, Prinja D, Jones D, Davies C A
Department of Pathology, University of Manchester.
Neuropathol Appl Neurobiol. 1990 Feb;16(1):17-25. doi: 10.1111/j.1365-2990.1990.tb00928.x.
The prevalence and severity of senile plaque (SP) formation was investigated in the cerebral cortex, hippocampus and amygdala of 60 non-demented individuals of age range 6-84 years, using immunocytochemical (anti-A4 amyloid, anti-PHF protein), lectin histochemical (Con A binding) and silver (Methenamine (MS) and Palmgren), staining methods. By at least one of these methods, 18 patients showed the presence of SP within one or more of these brain regions; 15 of these patients were over 60 years of age. Comparisons between each staining method showed that, in the hippocampus and amygdala all five methods detected the presence and number of SP equally well, whereas, in the cerebral cortex, MS and anti-A4 staining demonstrated more SP in a greater number of patients than did either Con A or Palmgren silver and anti-PHF staining. The additional SP detected by these former two staining methods contained diffuse deposits of amyloid (A4) protein, and sometimes also large clumps of Con A positive material, but no neurites as detected by Palmgren or anti-PHF staining. Such SP closely resemble those seen in the cerebral cortex of young patients with Down's syndrome, and which are thought to be an early form of SP. The relationship between the pathological changes in these non-demented patients and a possible diagnosis of early Alzheimer's disease is discussed.
采用免疫细胞化学方法（抗A4淀粉样蛋白、抗PHF蛋白）、凝集素组织化学方法（伴刀豆球蛋白A结合）以及银染法（六胺银（MS）和帕尔姆格伦银染法），对60名年龄在6至84岁之间的非痴呆个体的大脑皮质、海马体和杏仁核中老年斑（SP）形成的患病率和严重程度进行了研究。通过这些方法中的至少一种，18名患者在这些脑区中的一个或多个区域显示存在SP；其中15名患者年龄超过60岁。对每种染色方法的比较表明，在海马体和杏仁核中，所有五种方法在检测SP的存在和数量方面效果相同，而在大脑皮质中，MS和抗A4染色在更多患者中检测到的SP比伴刀豆球蛋白A或帕尔姆格伦银染法以及抗PHF染色更多。通过前两种染色方法检测到的额外SP包含淀粉样蛋白（A4）的弥漫性沉积物，有时还包含大量伴刀豆球蛋白A阳性物质，但未检测到帕尔姆格伦或抗PHF染色所显示的神经突。这种SP与唐氏综合征年轻患者大脑皮质中所见的SP非常相似，被认为是SP的早期形式。讨论了这些非痴呆患者的病理变化与早期阿尔茨海默病可能诊断之间的关系。
