戊二醛固定后经冷冻置换法改善深海微生物精细结构的保存。
Improved preservation of fine structure of deep-sea microorganisms by freeze-substitution after glutaraldehyde fixation.
作者信息
Yamaguchi Masashi, Namiki Yuichi, Okada Hitoshi, Uematsu Katsuyuki, Tame Akihiro, Maruyama Tadashi, Kozuka Yoshimichi
机构信息
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
出版信息
J Electron Microsc (Tokyo). 2011;60(4):283-7. doi: 10.1093/jmicro/dfr032. Epub 2011 May 13.
A method was proposed for improving preservation of ultrastructures of deep-sea microorganisms by using rapid-freeze freeze-substitution after glutaraldehyde fixation. This method produced clear high-resolution images of cells appearing in their natural state, close to the quality of images obtained by rapidly freezing freeze-substituted specimens of living cells. The method may be useful for observing any microorganism when rapid freezing of living samples is difficult and only glutaraldehyde fixation can be carried out.
提出了一种在戊二醛固定后使用快速冷冻冷冻置换来改善深海微生物超微结构保存的方法。该方法产生了细胞呈现自然状态的清晰高分辨率图像，接近通过快速冷冻冷冻置换活细胞标本所获得图像的质量。当活样品难以快速冷冻且只能进行戊二醛固定时，该方法可能有助于观察任何微生物。
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