通过冷冻置换固定更好地保存培养巨噬细胞和吞噬酵母细胞精细结构的简便方法。

Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation.

作者信息

Yamaguchi Masashi, Takahashi-Nakaguchi Azusa, Aida Yuko, Sato-Okamoto Michiyo, Chibana Hiroji

机构信息

Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku Chiba 260-8673,Japan.

出版信息

Microscopy (Oxf). 2017 Jun 1;66(3):209-211. doi: 10.1093/jmicro/dfx006.

DOI:10.1093/jmicro/dfx006

PMID:28339650

Abstract

Rapid freeze-freeze substitution after glutaraldehyde fixation (CF-FS method) obtained the natural and fine structures of macrophages and engulfed yeast cells. Culturing macrophages on single hole molybdenum grids placed in culture dishes made possible the rapid freezing of cells by the 'open sandwich method'. This method may be convenient when rapid-freezing cannot be performed immediately, or when a rapid-freezing device is not available in the lab.

摘要

戊二醛固定后进行快速冷冻替代（CF-FS法）可获得巨噬细胞和被吞噬酵母细胞的自然精细结构。将巨噬细胞培养在置于培养皿中的单孔钼网上，通过“开放式三明治法”可实现细胞的快速冷冻。当无法立即进行快速冷冻或实验室没有快速冷冻设备时，这种方法可能会很方便。