Scc1（CP0432）和 Scc4（CP0033）作为 Chlamydia pneumoniae 中 CopN 的 III 型分泌蛋白的分子伴侣。
Scc1 (CP0432) and Scc4 (CP0033) function as a type III secretion chaperone for CopN of Chlamydia pneumoniae.
机构信息
Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, Florida, USA.
出版信息
J Bacteriol. 2011 Jul;193(14):3490-6. doi: 10.1128/JB.00203-11. Epub 2011 May 13.
Abstract
The Chlamydia pneumoniae CopN protein is a member of the YopN/TyeA/InvE/MxiC family of secreted proteins that function to regulate the secretion of type III secretion system (T3SS) translocator and effector proteins. In this study, the Scc1 (CP0432) and Scc4 (CP0033) proteins of C. pneumoniae AR-39 were demonstrated to function together as a type III secretion chaperone that binds to an N-terminal region of CopN. The Scc1/Scc4 chaperone promoted the efficient secretion of CopN via a heterologous T3SS, whereas, the Scc3 chaperone, which binds to a C-terminal region of CopN, reduced CopN secretion.
摘要
肺炎衣原体 CopN 蛋白是 YopN/TyeA/InvE/MxiC 家族分泌蛋白的成员，该家族蛋白的功能是调节 III 型分泌系统 (T3SS) 转位器和效应蛋白的分泌。在本研究中，肺炎衣原体 AR-39 的 Scc1（CP0432）和 Scc4（CP0033）蛋白被证明作为一种 III 型分泌伴侣共同发挥作用，该伴侣与 CopN 的 N 端区域结合。Scc1/Scc4 伴侣通过异源 T3SS 促进 CopN 的有效分泌，而与 CopN 的 C 端区域结合的 Scc3 伴侣则减少 CopN 的分泌。
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