Department of Zoology, University of Melbourne, Melbourne, Australia.
Hum Reprod. 2011 Aug;26(8):1981-6. doi: 10.1093/humrep/der143. Epub 2011 May 13.
The aim of this study was to determine the relationship between nutrient utilization by the human embryo and its subsequent viability after transfer. METHODS The embryos of 50 patients having single blastocyst transfer were cultured individually from Day 3 in 10 µl drops of medium G2 under Ovoil in 5%O(2), 6%CO(2), 89%N(2). Patient inclusion in the study was maternal age ≤ 38. Embryos were moved to fresh drops of medium every 24 h. Spent media samples, including controls containing no embryo, were coded, frozen and subsequently analysed blind. Analysis of glucose was performed by microfluorimetry. The sex of children born was recorded.
Clinical pregnancy and live birth rates were 58 and 56%, respectively. Glucose consumption by embryos which resulted in a pregnancy was significantly higher on both Day 4 and Day 5 than that by embryos which failed to develop post-transfer (P < 0.01). Furthermore, on Day 4 female embryos consumed 28% more glucose compared with males (P < 0.05). Glucose uptake was independent of embryo grade.
The rapid screening of glucose metabolism by the human embryo on Day 4 and 5 may prove to be a useful metric in the development of algorithms for the selection of embryos for transfer in human IVF. Also, the observed sex-related metabolic difference provides preliminary data to support the hypothesis that male and female human embryos differ in their physiology due to the presence of two active X chromosomes and an altered proteome for a finite time during the preimplantation period.
本研究旨在确定人类胚胎对营养物质的利用与其随后移植后的活力之间的关系。
对 50 名接受单个囊胚移植的患者的胚胎进行研究，这些胚胎从第 3 天开始在含有 5%O(2)、6%CO(2)、89%N(2)的 Ovoil 中在 10 µl 培养液滴中单独培养。患者纳入研究的标准为年龄≤38 岁。胚胎每 24 小时转移到新鲜的培养液滴中。将含有胚胎的培养液样本与不含胚胎的对照样本编码、冷冻，然后进行盲法分析。通过微荧光法分析葡萄糖。记录出生儿童的性别。
临床妊娠率和活产率分别为 58%和 56%。在第 4 天和第 5 天，导致妊娠的胚胎的葡萄糖消耗明显高于移植后未发育的胚胎（P<0.01）。此外，第 4 天，雌性胚胎的葡萄糖摄取量比雄性胚胎高 28%（P<0.05）。葡萄糖摄取与胚胎等级无关。
人类胚胎在第 4 天和第 5 天快速筛选葡萄糖代谢可能成为人类体外受精胚胎选择转移算法开发的有用指标。此外，观察到的与性别相关的代谢差异为假设提供了初步数据，即由于两个活跃的 X 染色体的存在以及在植入前时期的有限时间内蛋白质组的改变，男性和女性人类胚胎在生理学上存在差异。
