VU University Medical Center, Reproductive Medicine, Department of Obstetrics and Gynaecology, IVF Center, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands.
Reprod Biomed Online. 2011 Dec;23(6):769-76. doi: 10.1016/j.rbmo.2011.08.015. Epub 2011 Sep 16.
This study investigated if metabolomic profiling of culture media using near infrared (NIR) spectroscopy was related to live-birth rates after single-embryo transfer of frozen-thawed embryos. Analysis of culture media of frozen-thawed embryos was performed by NIR spectroscopy. A viability score was calculated using a predictive multivariate algorithm of fresh day-5 embryos with known pregnancy outcomes. This algorithm generated with fresh day-5 embryos could help to identify the live-birth group from the no live-birth group. Multivariable regression models that tested the predictive ability of the viability score for live birth showed an odds ratio in the crude analysis of 1.50 (P=0.008), after adjustment for embryo morphology, 1.44 (P=0.022), and after adjustment for all variables, 1.71 (P=0.005); based on a 0.1 step increase in viability scores. In conclusion, higher viability scores resulted in higher live-birth rates. An algorithm generated from fresh embryos might be used to predict viability of frozen-thawed embryos. Frozen-thawed embryos have different metabolic activity which is related to implantation potential. Therefore, this method might be useful to select the best embryo for transfer within a group of embryos with similar morphology.
本研究探讨了使用近红外（NIR）光谱对冷冻-解冻胚胎的培养介质进行代谢组学分析是否与单胚胎移植后的活产率相关。通过 NIR 光谱对冷冻-解冻胚胎的培养介质进行分析。使用具有已知妊娠结局的新鲜第 5 天胚胎的预测多元算法计算生存能力评分。该算法可帮助识别无活产组中的活产组。多元回归模型测试了生存能力评分对活产的预测能力，结果显示，在未调整胚胎形态的情况下，粗分析中的优势比为 1.50（P=0.008），调整胚胎形态后为 1.44（P=0.022），调整所有变量后为 1.71（P=0.005）；基于生存能力评分增加 0.1 步。总之，较高的生存能力评分导致较高的活产率。从新鲜胚胎生成的算法可用于预测冷冻-解冻胚胎的活力。冷冻-解冻胚胎具有不同的代谢活性，这与植入潜力有关。因此，该方法可能有助于在形态相似的胚胎组中选择最佳的胚胎进行移植。
