Christensen John Mark, Chuong Monica Chang, Le Hang, Pham Loan, Bendas Ehab
Pharmaceutical Sciences, College of Pharmacy, Oregon State University Corvallis, OR, USA.
Arch Drug Inf. 2011 Mar;4(1):10-21. doi: 10.1111/j.1753-5174.2010.00033.x.
The penetration of hydrocortisone (HC) from six topical over-the-counter products along with one prescription cream through cultured normal human-derived epidermal keratinocytes (Epiderm™), mouse skin and synthetic nylon membrane was performed as well as the effect hydrating the skin by pre-washing was explored using the Upright Franz Cell. METHOD AND RESULTS: Permeation of HC through EpiDerm™, mouse skin and synthetic membrane was highest with the topical HC gel formulation with prewash treatment of the membranes among seven products evaluated, 198 ± 32 µg/cm(2), 746.32 ± 12.43 µg/cm(2), and 1882 ± 395.18 µg/cm(2), respectively. Pre-washing to hydrate the skin enhanced HC penetration through EpiDerm™ and mouse skin. The 24-hour HC released from topical gel with prewash treatment was 198.495 ± 32 µg/cm(2) and 746.32 ± 12.43 µg/cm(2) while without prewash, the 24-h HC released from topical gel was 67.2 ± 7.41 µg/cm(2) and 653.43 ± 85.62 µg/cm(2) though EpiDerm™ and mouse skin, respectively. HC penetration through synthetic membrane was ten times greater than through mouse skin and EpiDerm™. Generally, the shape, pattern, and rank order of HC diffusion from each commercial product was similar through each membrane.
使用直立式Franz扩散池，对六种非处方外用产品和一种处方乳膏中的氢化可的松（HC）透过培养的正常人源表皮角质形成细胞（Epiderm™）、小鼠皮肤和合成尼龙膜的渗透情况进行了研究，并探讨了预洗对皮肤保湿的影响以及对HC渗透的作用。方法与结果：在所评估的七种产品中，经膜预洗处理的外用HC凝胶制剂中，HC透过Epiderm™、小鼠皮肤和合成膜的渗透率最高，分别为198±32μg/cm²、746.32±12.43μg/cm²和1882±395.18μg/cm²。预洗使皮肤保湿可增强HC透过Epiderm™和小鼠皮肤的渗透。经预洗处理的外用凝胶24小时释放的HC量为198.495±32μg/cm²和746.32±12.43μg/cm²，而未经预洗的外用凝胶透过Epiderm™和小鼠皮肤24小时释放的HC量分别为67.2±7.41μg/cm²和653.43±85.62μg/cm²。HC透过合成膜的渗透率比透过小鼠皮肤和Epiderm™高十倍。一般来说，每种市售产品中HC通过各膜的扩散形状、模式和排序相似。
