Department of Pharmacology, M.V.P. Samaj's College of Pharmacy, Gangapur Road, Nashik, Maharashtra, India.
Indian J Pharmacol. 2011 Apr;43(2):137-42. doi: 10.4103/0253-7613.77347.
The objective of the present study was to evaluate the cognitive enhancing and antioxidant activity of Hibiscus rosa sinensis.
The learning and memory was impaired by administration of scopolamine (1 mg/kg, i.p.) in mice which is associated with altered brain oxidative status. The object recognition test (ORT) and passive avoidance test (PAT) were used to assess cognitive enhancing activity. Animals were treated with an ethyl acetate soluble fraction of the methanol extract of H. sinensis (25, 50 and 100 mg/kg, p.o).
The ethyl acetate soluble fraction of the methanol extract of H. sinensis (EASF) attenuated amnesia induced by scopolamine and aging. The discrimination index (DI) was significantly decreased in the aged and scopolamine group in ORT. Pretreatment with EASF significantly increased the DI. In PAT, scopolamine-treated mice exhibited significantly shorter step-down latencies (SDL). EASF treatment showed a significant increase in SDL in young, aged as well as in scopolamine-treated animals. The biochemical analysis of brain revealed that scopolamine treatment increased lipid peroxidation and decreased levels of superoxide dismutase (SOD) and glutathione reductase (GSH). Administration of extract significantly reduced LPO and reversed the decrease in brain SOD and GSH levels. The administration of H. sinensis improved memory in amnesic mice and prevented the oxidative stress associated with scopolamine. The mechanism of such protection of H. sinensis may be due to augmentation of cellular antioxidants.
The results of the present study suggested that H. sinensis had a protective role against age and scopolamine-induced amnesia, indicating its utility in management of cognitive disorders.
本研究旨在评估木槿的认知增强和抗氧化活性。
通过给予小鼠东莨菪碱（1mg/kg，ip）来损害学习和记忆，东莨菪碱与大脑氧化状态改变有关。使用物体识别测试（ORT）和被动回避测试（PAT）来评估认知增强活性。动物用木槿甲醇提取物的乙酸乙酯可溶部分（25、50 和 100mg/kg，po）进行治疗。
木槿甲醇提取物的乙酸乙酯可溶部分（EASF）可减轻东莨菪碱和衰老引起的健忘症。在 ORT 中，年龄和东莨菪碱组的辨别指数（DI）显着降低。EASF 预处理显着增加了 DI。在 PAT 中，东莨菪碱处理的小鼠表现出明显较短的跳下潜伏期（SDL）。EASF 治疗在年轻、年老和东莨菪碱处理的动物中均显着增加 SDL。大脑的生化分析显示，东莨菪碱处理增加了脂质过氧化作用，并降低了超氧化物歧化酶（SOD）和谷胱甘肽还原酶（GSH）的水平。提取物的给药显着降低了 LPO，并逆转了大脑 SOD 和 GSH 水平的降低。木槿的给药改善了健忘症小鼠的记忆，并防止了与东莨菪碱相关的氧化应激。木槿具有这种保护作用的机制可能是由于细胞抗氧化剂的增加。
本研究的结果表明，木槿对年龄和东莨菪碱引起的健忘症具有保护作用，表明其在认知障碍管理中的应用。
