Laboratory of Bioanalytical Chemistry, Toxicology and Applied Physical Chemistry, Institute of Pharmacy, Free University of Brussels, Campus de la Plaine, CP 205/01, Boulevard du Triomphe, B-1050 Brussels, Belgium.
Int J Mol Med. 2011 Aug;28(2):261-70. doi: 10.3892/ijmm.2011.695. Epub 2011 May 9.
Agelanthus dodoneifolius DC Danser (Loranthaceae) is used for the treatment of various diseases including asthma. The aqueous and hydroalcoholic extracts have been reported to have anti-inflammatory, spasmolytic and bronchorelaxant activities. The present study investigates the effects of the aqueous decoction and the diethyl ether, ethyl acetate and butanolic fractions of Agelanthus dodoneifolius DC Danser (Loranthaceae) on reactive oxygen species (ROS) production and myeloperoxidase (MPO) release by phorbol 12-myristate 13-acetate (PMA)-stimulated equine neutrophils and on purified equine MPO activity. ROS production and MPO release by the PMA-stimulated neutrophils were measured by the lucigenin-enhanced chemiluminescence and ELISA assays, respectively. Specific immunological extraction followed by enzymatic detection (SIEFED) was used to specifically measure the equine MPO activity. Identification and quantification of the individual and total phenolic and flavonoid compounds were performed using UPLC-MS/MS equipment and colorimetric methods involving Folin-Ciocalteu and AlCl₃, respectively. All the tested extracts displayed dose-dependent inhibitory effects on the oxidant activities of neutrophils; a stronger effect was observed with the organic fractions than the aqueous decoction. These findings could be correlated with a high content of phenolic and flavonoid compounds. The results confirm the previously shown anti-inflammatory effect of Agelanthus dodoneifolius and its potential use for the treatment of neutrophil-dependent inflammatory diseases.
醉鱼草（醉鱼草科）的水煎剂和正丁醇、乙酸乙酯和二乙醚部位被报道具有抗炎、松弛平滑肌和支气管舒张活性。本研究调查了醉鱼草（醉鱼草科）的水煎剂和正丁醇、乙酸乙酯和二乙醚部位对佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）刺激的马嗜中性粒细胞产生活性氧物种（ROS）和髓过氧化物酶（MPO）释放的影响，以及对纯化的马 MPO 活性的影响。通过荧光素增强化学发光和 ELISA 测定分别测量 PMA 刺激的嗜中性粒细胞产生的 ROS 产生和 MPO 释放。使用特异性免疫提取后酶检测（SIEFED）特异性测量马 MPO 活性。使用 UPLC-MS/MS 设备和涉及福林-希考尔特和 AlCl₃的比色法分别对个体和总酚类和类黄酮化合物进行鉴定和定量。所有测试的提取物均显示出对嗜中性粒细胞氧化活性的剂量依赖性抑制作用；与水煎剂相比，有机部位的作用更强。这些发现可以与高含量的酚类和类黄酮化合物相关。研究结果证实了醉鱼草先前显示的抗炎作用及其在治疗嗜中性粒细胞依赖性炎症性疾病方面的潜在用途。
