Kohnen Stephan, Franck Thierry, Van Antwerpen Pierre, Boudjeltia Karim Zouaoui, Mouithys-Mickalad Ange, Deby Carol, Moguilevsky Nicole, Deby-Dupont Ginette, Lamy Maurice, Serteyn Didier
Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine B41, University of Liège, Sart Tilman, 4000 Liège, Belgium.
J Agric Food Chem. 2007 Oct 3;55(20):8080-7. doi: 10.1021/jf071741n. Epub 2007 Sep 11.
Resveratrol is a polyphenolic antioxidant present in beverage and food known for its multiple protective effects. We report the inhibitory effects of resveratrol on equine myeloperoxidase (MPO), a hemic peroxidase present in the granules of the neutrophils involved in the inflammatory response. Resveratrol inhibited the production of reactive oxygen species (ROS) by stimulated equine neutrophils by acting as a direct scavenger of the ROS released by the cells but did not modify the degranulation of the stimulated neutrophils as the amounts of released MPO were unchanged. Resveratrol strongly inhibited the chlorination, oxidation, and nitration activities of MPO in a dose-dependent manner. By an original technique of specific immunological extraction followed by enzymatic detection (SIEFED), we demonstrated that resveratrol inhibited the peroxidasic activity of the MPO measured by a direct interaction such as the fixation of resveratrol on the enzyme. The observation of a decrease of the accumulation of compound II suggested that resveratrol acts as an electron donor for MPO reduction.
白藜芦醇是一种存在于饮料和食物中的多酚类抗氧化剂，因其多种保护作用而闻名。我们报告了白藜芦醇对马髓过氧化物酶（MPO）的抑制作用，MPO是一种存在于参与炎症反应的中性粒细胞颗粒中的血红素过氧化物酶。白藜芦醇通过作为细胞释放的活性氧（ROS）的直接清除剂，抑制了受刺激的马中性粒细胞产生ROS，但由于释放的MPO量未改变，因此并未改变受刺激中性粒细胞的脱颗粒情况。白藜芦醇以剂量依赖的方式强烈抑制MPO的氯化、氧化和硝化活性。通过一种特定免疫提取后酶检测（SIEFED）的原始技术，我们证明白藜芦醇通过直接相互作用（如白藜芦醇与酶的结合）抑制了所测MPO的过氧化物酶活性。化合物II积累的减少表明白藜芦醇作为电子供体参与MPO的还原。
