谷胱甘肽还原酶发挥钒酸盐（V）还原酶的作用。

Glutathione reductase functions as vanadate(V) reductase.

作者信息

Shi X L, Dalal N S

机构信息

Department of Chemistry, West Virginia University, Morgantown 26505.

出版信息

Arch Biochem Biophys. 1990 Apr;278(1):288-90. doi: 10.1016/0003-9861(90)90262-w.

DOI:10.1016/0003-9861(90)90262-w

PMID:2157361

Abstract

The oxidation of NADPH by vanadate(V) in the presence of glutathione reductase showed typical enzymatic kinetics. The oxidation was inhibited by N-ethylmaleimide, a glutathione reductase inhibitor. Superoxide dismutase had no significant effect on the oxidation, indicating noninvolvement of the superoxide radical. The vanadate(V) reduction was found to be a one-electron transfer process. These results suggest a new pathway for vanadate(V) metabolism and a new function of glutathione reductase.

摘要

在谷胱甘肽还原酶存在的情况下，钒酸盐（V）对NADPH的氧化呈现出典型的酶促动力学。该氧化反应受到谷胱甘肽还原酶抑制剂N-乙基马来酰亚胺的抑制。超氧化物歧化酶对该氧化反应无显著影响，表明超氧阴离子未参与其中。钒酸盐（V）的还原是一个单电子转移过程。这些结果提示了钒酸盐（V）代谢的一条新途径以及谷胱甘肽还原酶的一项新功能。