Ko Yongseok, Kang Saehyun, Yang Youri, Lee Jisu, Hur Hor-Gil
School of Environment and Energy Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
Department of Physics and Photon Science, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
J Microbiol Biotechnol. 2025 May 26;35:e2502051. doi: 10.4014/jmb.2502.02051.
Vanadium dioxide (VO) nanoparticles have various application potentials such as smart windows and electronic devices due to their unique phase transition properties. However, conventional VO synthesis methods require harsh conditions and toxic reducing agents, leading to environmental problems. In this study, we developed an eco-friendly method to biosynthesize VO nanoparticles using sp. strain HN-41 under anaerobic conditions at 30°C and neutral pH. Morphological observations revealed that biogenic VO nanoparticles with an average size of 4.3 nm were in the form of granules presented inside and outside the cells. These nanoparticles were identified as VO by differential scanning calorimetry (DSC) analysis, which showed a phase transition temperature of 61.9°C, consistent with that of VO. Furthermore, we observed an active formation of vesicles containing VO nanoparticles by the cross-sectioned transmission electron microscopy (TEM) analysis. Thus, in addition to the direct extracellular formation of VO nanoparticles through anaerobic respiration, bacterial membrane vesicles likely play a role in expelling nanoparticles from the cell, potentially mitigating their toxicity. These findings highlight metal reducing bacteria could be a biological green agent for the production of valuable VO nanoparticles under anaerobic environmental conditions.
二氧化钒（VO）纳米颗粒由于其独特的相变特性，在智能窗户和电子设备等方面具有多种应用潜力。然而，传统的VO合成方法需要苛刻的条件和有毒的还原剂，从而导致环境问题。在本研究中，我们开发了一种生态友好的方法，利用sp.菌株HN - 41在30°C和中性pH的厌氧条件下生物合成VO纳米颗粒。形态学观察表明，平均尺寸为4.3 nm的生物源VO纳米颗粒呈颗粒状，存在于细胞内外。通过差示扫描量热法（DSC）分析确定这些纳米颗粒为VO，其显示的相变温度为61.9°C，与VO的相变温度一致。此外，通过横截面透射电子显微镜（TEM）分析，我们观察到含有VO纳米颗粒的囊泡的活跃形成。因此，除了通过厌氧呼吸在细胞外直接形成VO纳米颗粒外，细菌膜囊泡可能在将纳米颗粒排出细胞方面发挥作用，从而可能减轻其毒性。这些发现突出表明，金属还原细菌可能是在厌氧环境条件下生产有价值的VO纳米颗粒的生物绿色剂。
