从同源和异源饲养细胞的体外生产的囊胚中分离水牛胚胎干细胞样细胞。
Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells.
机构信息
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, 132001, India.
出版信息
J Assist Reprod Genet. 2011 Aug;28(8):679-88. doi: 10.1007/s10815-011-9572-2. Epub 2011 May 4.
PURPOSE
The aim of the present study is to compare the ability of homologous and heterologous embryonic fibroblast feeder layers to support isolation and proliferation of buffalo ES-like cells generated from hatched and expanded blastocysts produced by in vitro fertilization and characterization of derived cells through expression of pluripotent markers.
METHODS
Embryonic stem cells were derived from hatched and expanded blastocysts through intact blastocyst culture and enzymatic method respectively and compared for proliferation rate on homologous (buffalo) and heterologous feeder layers (goat and sheep).
RESULTS
A total of 69 hatched and 83 expanded blastocysts were used for isolation of inner cell masses which were seeded on buffalo, goat and sheep embryonic feeder layers. Following seeding, attachment rate, primary colony formation rate and survival to maximum number of passages were observed to be higher on homologous feeder layers.
CONCLUSIONS
Upon comparison of different feeder layer cells for derivation and maintenance of buffalo ES-like cells from hatched and expanded blastocysts, buffalo embryonic fibroblast cells were able to provide a better environment for maintaining pluripotency in culture conditions.
目的
本研究旨在比较同源和异源胚胎成纤维细胞饲养层支持从体外受精产生的孵化和扩张囊胚中分离和增殖水牛类 ES 细胞的能力，并通过多能性标记物的表达对衍生细胞进行特征分析。
方法
通过完整囊胚培养和酶法分别从孵化和扩张囊胚中获得胚胎干细胞，并比较其在同源（水牛）和异源（山羊和绵羊）饲养层上的增殖率。
结果
共使用了 69 个孵化和 83 个扩张囊胚来分离内细胞团，然后将其接种在水牛、山羊和绵羊胚胎饲养层上。接种后，在同源饲养层上观察到附着率、初代集落形成率和最大传代数的存活率更高。
结论
在比较不同饲养层细胞以从孵化和扩张囊胚中分离和维持水牛类 ES 细胞时，水牛胚胎成纤维细胞能够在培养条件下为维持多能性提供更好的环境。
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