Buffalo Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India.
Cytotechnology. 2013 Jan;65(1):23-30. doi: 10.1007/s10616-012-9464-z. Epub 2012 Jul 21.
Stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. We report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, OCT-4, NANOG and SOX-2) from the amnion of water buffalo (Bubalus bubalis). The cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. The cytochemical staining revealed that pluripotent cells were capable of undergoing directed differentiation in vitro into osteocytes. It could be inferred that amnion-derived pluripotent stem cell-like cells can be isolated, cultured for many passages and differentiated into mesoderm lineage, and may be an alternative source to mesenchymal stem cells. These cells can have applications in assisted reproduction, developmental biological and regenerative medicine.
干细胞在畜牧业辅助生殖和兽医治疗领域（如组织工程）中是一种重要的工具。我们首次报道了从水牛（Bubalus bubalis）的羊膜中分离出具有多能性标记物（碱性磷酸酶、OCT-4、NANOG 和 SOX-2）的多能干细胞样细胞。这些细胞在冷冻保存前后的染色体图谱中没有明显异常。细胞化学染色显示，多潜能细胞能够在体外定向分化为成骨细胞。可以推断，羊膜来源的多能干细胞样细胞可以被分离、培养多代，并分化为中胚层谱系，可能是间充质干细胞的替代来源。这些细胞可应用于辅助生殖、发育生物学和再生医学。
