University of Liège-Gembloux Agro-Bio Tech., Unit of Tropical Crop Husbandry and Horticulture, Gembloux Agricultural University, Passage des Déportés 2, 5030 Gembloux, Belgium.
Mol Biol Rep. 2012 Feb;39(2):1133-43. doi: 10.1007/s11033-011-0842-y. Epub 2011 May 15.
A full-length cDNA encoding common bean (Phaseolus vulgaris L.) sucrose synthase (designated as Pv_BAT93 Sus), which catalyses the synthesis and cleavage of sucrose, was isolated from seeds at 15 days after pollination (DAP) by rapid amplification of cDNA ends (RACE). The full-length cDNA of Pv_BAT93 Sus had a 2,418 bp open reading frame (ORF) encoding a protein of 806 amino acid residues. Sequence comparison analysis showed that Pv_BAT93 Sus was very similar to several members of the sucrose synthase family of other plant species. Tissue expression pattern analysis showed that Pv_BAT93 Sus was expressed in leaves, flowers, stems, roots, cotyledons, and particularly during seed development. Expression studies using in situ hybridization revealed altered spatial and temporal patterns of Sus expression in the EMS mutant relative to wild-type and confirmed Sus expression in common bean developing seeds. The expression and accumulation of Sus mRNA was clearly shown in several tissues, such as the suspensor and embryo, but also in the transfer cells and endothelium. The results highlight the diverse roles that Sus might play during seed development in common bean.
从授粉后 15 天的种子中，通过快速扩增 cDNA 末端（RACE）技术分离出全长 cDNA 编码普通豆（Phaseolus vulgaris L.）蔗糖合酶（命名为 Pv_BAT93 Sus），该酶催化蔗糖的合成和裂解。Pv_BAT93 Sus 的全长 cDNA 具有 2418bp 的开放阅读框（ORF），编码 806 个氨基酸残基的蛋白质。序列比较分析表明，Pv_BAT93 Sus 与其他植物物种的蔗糖合酶家族的几个成员非常相似。组织表达模式分析表明，Pv_BAT93 Sus 在叶片、花朵、茎、根、子叶中表达，特别是在种子发育过程中表达。原位杂交表达研究表明，相对于野生型和证实的普通豆发育种子中的 Sus 表达，EMS 突变体中 Sus 的表达具有改变的时空模式。Sus mRNA 的表达和积累在几种组织中，如悬浮细胞和胚胎中，也在转移细胞和内皮中表达。这些结果突出表明，Sus 在普通豆种子发育过程中可能发挥多种作用。
