Nanjing Sub-Center (Rapeseed) of National Center of Oilseeds Crop Improvement, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Mol Biol Rep. 2009 Nov;36(8):2375-86. doi: 10.1007/s11033-009-9460-3. Epub 2009 Feb 15.
The regulation of seed oil synthesis in rapeseed is largely unknown. In this study, Arabidopsis microarray was used to analyze the gene differential expression of the immature seeds 30 days after flowering of a high oil Brassica napus, H105, whose oil content was 46.04 +/- 1.42, 53.94 +/- 1.35 and 53.09 +/- 1.35% when planted in Nanjing (altitude: 8.9 m), Xining (altitude: 2261.2 m) and Lhasa (altitude: 3658 m), respectively. Transcript levels of 363 genes and 421 genes were altered twofold or more for H105 planted in Xining and Lhasa compared to that in Nanjing, respectively. Together, there were 53 common up-regulated and 42 common down-regulated expression transcripts shared by H105 planted in Xining and Lhasa compared to that in Nanjing. Some important genes, such as sucrose synthase, pyruvate kinase and 6-phosphogluconate dehydrogenase which related to sugar metabolism were identified common up-regulated in higher oil content H105. These results revealed the expressional disciplinarian of correlative genes, and provided important information of the molecular genetic mechanism of oil content difference of rapeseed. In addition, these differential expression genes could be suitable as targets for genetic improvement of seed oil content.
油菜籽中油脂合成的调控机制很大程度上仍是未知的。本研究利用拟南芥基因芯片分析了高油油菜品种‘H105’开花后 30 天的未成熟种子的基因差异表达情况，该品种在南京（海拔 8.9 米）、西宁（海拔 2261.2 米）和拉萨（海拔 3658 米）的含油率分别为 46.04%±1.42%、53.94%±1.35%和 53.09%±1.35%。与在南京种植的 H105 相比，在西宁和拉萨种植的 H105 分别有 363 个和 421 个基因转录水平发生了两倍或两倍以上的变化。共有 53 个上调表达和 42 个下调表达的共同转录本在西宁和拉萨种植的 H105 中被共同上调。鉴定出一些与糖代谢相关的重要基因，如蔗糖合酶、丙酮酸激酶和 6-磷酸葡萄糖酸脱氢酶，在高油含量的 H105 中共同上调。这些结果揭示了相关基因的表达规律，为油菜籽含油量差异的分子遗传机制提供了重要信息。此外，这些差异表达的基因可以作为遗传改良种子含油量的靶标。
