Andreasen J R, Brown J, Glisson J R, Villegas P
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30605.
Avian Dis. 1990 Jan-Mar;34(1):185-92.
A virus-neutralization test for infectious laryngotracheitis virus was performed in microtiter plates using standard techniques. To assess the reproducibility of the test, 11 sera were each titrated repeatedly once a week for 8 weeks, and the results were compared to a standard. The standard used for reproducibility was that the 95% logarithmic confidence intervals of the mean calculated from three titrations of the same serum had to be smaller than the logarithmic distance "within" two microtiter plate wells. For the virus neutralization test to give reproducible results, such confidence intervals had to fall "within" two wells at least 75% of the time. Over the 8 weeks, percent reproducibility varied from 43.5% to 81.5%. The infectious laryngotracheitis virus-neutralization test did not meet our defined standard of reproducibility with positive antisera. Results with negative control sera were reproducible, however. Percent reproducibility varied from 31.8% to 93.8% for different sera tested, but it was not related to the titer of the sera.
采用标准技术在微量滴定板中对传染性喉气管炎病毒进行病毒中和试验。为评估该试验的可重复性，每周对11份血清重复滴定一次，共进行8周，并将结果与标准进行比较。用于评估可重复性的标准是，同一血清三次滴定计算所得均值的95%对数置信区间必须小于两个微量滴定板孔之间的对数距离。为使病毒中和试验得出可重复的结果，此类置信区间必须至少75%的时间落在两个孔“之内”。在8周期间，可重复性百分比从43.5%到81.5%不等。传染性喉气管炎病毒中和试验对于阳性抗血清未达到我们定义的可重复性标准。然而，阴性对照血清的结果具有可重复性。不同测试血清的可重复性百分比从31.8%到93.8%不等，但与血清滴度无关。
