Stead S L, Wolodko-Cierniak K B, Richmond S F, Sharman M, Driver P, Teale P, Leonardova O, Purvis D
a The Food and Environment Research Agency (Fera) , Sand Hutton , York YO41 1LZ , UK.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011;28(7):848-59. doi: 10.1080/19440049.2011.567399. Epub 2011 May 13.
A potentiometric biosensor assay based on a commercially available polyclonal antibody was developed to detect tylosin residues in animal feed. The method can be used as a rapid (less than 45 min) laboratory-based procedure or as a portable field-test for the simultaneous measurement of up to 12 different samples. For both procedures the qualitative detection capability (CCβ) for tylosin was determined as 0.2 mg kg(-1) in a range of animal feeds with a measurement repeatability at concentrations between 0.2 and 4 mg kg(-1) of ≤13% coefficient of variation (%CV). The field-test format was capable of detecting tylosin residues at operating (external air) temperatures ranging between +4 and 37°C, although some reduction in signal was observed at the lower temperatures. The laboratory-based tylosin assay was evaluated using 16 medicated and 22 non-medicated feeds and was found to give comparable data with a confirmatory method based upon liquid chromatography-tandem mass spectrometry (LC-MS/MS). The potential to develop a multi-probe format assay for the simultaneous detection of tylosin, spiramycin and virginiamycin was also demonstrated. Cross-validation in a second laboratory showed the assay to be transferable, reliable and robust.
基于市售多克隆抗体开发了一种电位生物传感器检测方法，用于检测动物饲料中的泰乐菌素残留。该方法既可以作为一种快速（少于45分钟）的基于实验室的检测程序，也可以作为一种便携式现场检测方法，用于同时测量多达12个不同的样品。对于这两种检测程序，在一系列动物饲料中，泰乐菌素的定性检测能力（CCβ）被确定为0.2 mg kg⁻¹，在0.2至4 mg kg⁻¹的浓度范围内，测量重复性的变异系数（%CV）≤13%。现场检测形式能够在+4至37°C的操作（外部空气）温度范围内检测泰乐菌素残留，尽管在较低温度下观察到信号有所降低。使用16种加药饲料和22种未加药饲料对基于实验室的泰乐菌素检测方法进行了评估，发现其与基于液相色谱 - 串联质谱（LC-MS/MS）的确认方法给出的数据具有可比性。还展示了开发用于同时检测泰乐菌素、螺旋霉素和维吉尼亚霉素的多探针形式检测方法的潜力。在第二个实验室进行的交叉验证表明该检测方法具有可转移性、可靠性和稳健性。
