Lin Yulong, Wen Kun, Pan Yuxian, Wang Yadi, Che Xiaoyan, Wang Bin
Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China.
J Immunoassay Immunochem. 2011;32(3):233-43. doi: 10.1080/15321819.2011.559297.
To evaluate the cross-reactivity of anti-EV71 IgM and neutralizing antibody in series sera of patients infected with EV71 and CA16.
Real-time RT-PCR, virus isolation, ELISA and neutralization test were used to detect enteroviruses from clinical specimens and series sera of 79 HFMD patients.
27 EV71, 37 CA16, and 11 other enterovirus-infected patients were identified by RT-PCR. Among EV71 infected patients, anti-EV71 IgM positive ratios were 87.5% during 1-3 days after onset and 100% over 4 days after onset. In CA16 infected patients, the positive ratios were 7.4%, 26.4%, and 62.5% during 1-3 days, 4-6 days, and over 6 days after onset, respectively. Meanwhile, the results of neutralization test showed 18.9% of CA16 infected patients and 11.1% of EV71 infected patients present high cross-neutralization antibody against each other.
Cross-reactivity of anti-EV71 IgM in patients infected with EV71 and CA16 becomes stronger with the progress of disease. Moreover, high cross-neutralization antibody existing in part of patients suggests that the immune reactivity to EV71 infection can be recalled by CA16, and the immune reactivity to CA16 infection can be recalled by EV71. Therefore, identifying enteroviruses by neutralization test may not be an ideal selection.
评估肠道病毒71型（EV71）和柯萨奇病毒A16型（CA16）感染患者系列血清中抗EV71 IgM和中和抗体的交叉反应性。
采用实时逆转录聚合酶链反应（RT-PCR）、病毒分离、酶联免疫吸附测定（ELISA）和中和试验，检测79例手足口病（HFMD）患者临床标本及系列血清中的肠道病毒。
RT-PCR鉴定出27例EV71感染患者、37例CA16感染患者和11例其他肠道病毒感染患者。在EV71感染患者中，发病后1 - 3天抗EV71 IgM阳性率为87.5%，发病4天后为100%。在CA16感染患者中，发病后1 - 3天、4 - 6天和6天以上的阳性率分别为7.4%、26.4%和62.5%。同时，中和试验结果显示，18.9%的CA16感染患者和11.1%的EV71感染患者存在相互间的高交叉中和抗体。
EV71和CA16感染患者中抗EV71 IgM的交叉反应性随疾病进展增强。此外，部分患者存在高交叉中和抗体，提示CA16可唤起对EV71感染的免疫反应，EV71可唤起对CA16感染的免疫反应。因此，通过中和试验鉴定肠道病毒可能不是理想选择。
