地塞米松可增加3T3-L1成纤维细胞中的中性鞘磷脂酶活性和鞘氨醇水平。
Dexamethasone increases neutral sphingomyelinase activity and sphingosine levels in 3T3-L1 fibroblasts.
作者信息
Ramachandran C K, Murray D K, Nelson D H
机构信息
Department of Medicine, University of Utah School of Medicine, Salt Lake City 84132.
出版信息
Biochem Biophys Res Commun. 1990 Mar 16;167(2):607-13. doi: 10.1016/0006-291x(90)92068-b.
The activity of neutral sphingomyelinase (EC 3.1.4.12) in a plasma membrane enriched fraction was found to be increased in dexamethasone treated cells. The elevation of sphingomyelinase activity was blocked by cycloheximide indicating that protein synthesis was required for the steroid action. Ceramidase (EC3.5.1.23) activity was unaffected by the dexamethasone treatment. Levels of sphingosine in 3T3-L1 Cells were also increased after treatment with 10(-7) M dexamethasone for 2 and 4 hours.
在地塞米松处理的细胞中，发现富含质膜的部分中中性鞘磷脂酶（EC 3.1.4.12）的活性增加。环己酰亚胺可阻断鞘磷脂酶活性的升高，这表明类固醇作用需要蛋白质合成。神经酰胺酶（EC3.5.1.23）的活性不受地塞米松处理的影响。用10^(-7) M地塞米松处理3T3-L1细胞2小时和4小时后，鞘氨醇水平也升高。
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