大鼠子宫肌层膜中的一种高亲和力缓激肽受体与Gi家族中对百日咳毒素敏感的G蛋白偶联。
A high-affinity bradykinin receptor in membranes from rat myometrium is coupled to pertussis toxin-sensitive G-proteins of the Gi family.
作者信息
Liebmann C, Offermanns S, Spicher K, Hinsch K D, Schnittler M, Morgat J L, Reissmann S, Schultz G, Rosenthal W
机构信息
Bereich Allgemeine Biochemie, Friedrich-Schiller-Universität Jena, East Germany.
出版信息
Biochem Biophys Res Commun. 1990 Mar 30;167(3):910-7. doi: 10.1016/0006-291x(90)90610-y.
In rat myometrial membranes, two 3H-Bradykinin binding sites with KD values of 16 pM and 1.0 nM were identified. Employed at pM concentrations, bradykinin stimulated high affinity GTPases. This effect was abolished by the bradykinin antagonist, [D-Arg(Hyp3-Thi5,8, D-Phe7)]bradykinin (10 microM), and by treatment of membranes with pertussis toxin. Myometrial membranes contained two pertussis toxin substrates of 40 and 41 kDa, which corresponded immunologically to alpha-subunits of Gi-type G-proteins. The faster migrating substrate was tentatively identified as Gi2 alpha-subunit. The electrophoretic mobility of the slower migrating Gi alpha-subunit was very similar to that of the Gi3 alpha-subunit. Go alpha-subunits were not detected. Thus, in uterine smooth muscle, G-proteins of the Gi-family (Gi2, Gi3) couple high-affinity bradykinin receptors to their effector enzymes.
在大鼠子宫肌层膜中，鉴定出两个3H-缓激肽结合位点，其解离常数（KD）值分别为16 pM和1.0 nM。在pM浓度下使用时，缓激肽刺激高亲和力GTP酶。缓激肽拮抗剂[D-Arg（Hyp3-Thi5,8，D-Phe7）]缓激肽（10 microM）以及用百日咳毒素处理膜可消除这种效应。子宫肌层膜含有两种40 kDa和41 kDa的百日咳毒素底物，它们在免疫学上与Gi型G蛋白的α亚基相对应。迁移速度较快的底物初步鉴定为Gi2α亚基。迁移速度较慢的Giα亚基的电泳迁移率与Gi3α亚基非常相似。未检测到Goα亚基。因此，在子宫平滑肌中，Gi家族的G蛋白（Gi2、Gi3）将高亲和力缓激肽受体与其效应酶偶联。
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