Fan Li, Xu Yong, Lian Zhina, Yong Qiang, Yu Shiyuan
Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Se Pu. 2011 Jan;29(1):75-8. doi: 10.3724/sp.j.1123.2011.00075.
A method for the analysis of xylo-oligosaccharides (XOS) in xylo-oligosaccharide products, including xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose, was developed using high performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The retention times of xyloheptaose and xylooctaose were calculated according to the linear relationship between the retention time and the polymerization degree. The separation was performed on a CarboPac PA200 column (250 mm x 3 mm) with a gradient elution of NaOH-NaOAc as the mobile phase. The calibration curves showed good linearity for the xylo-oligosaccharides in the range of 0.804-8.607 mg/L. The detection limits (LODs) and the quantification limits (LOQs) were 0.064 -0.111 mg/L and 0.214 -0.371 mg/L, respectively. Under the optimized conditions, the recoveries of xylo-oligosaccharides at three different spiked levels ranged from 84.29%-118.19%, with the relative standard deviations (RSDs, n = 3) of 0.44%-14.87%. This method is fast and accurate for the quantitative analysis of the xylo-oligosaccharide products.
建立了一种采用高效阴离子交换色谱-脉冲安培检测法（HPAEC-PAD）分析低聚木糖产品中木二糖、木三糖、木四糖、木五糖和木六糖等低聚木糖（XOS）的方法。根据保留时间与聚合度之间的线性关系计算出木七糖和木八糖的保留时间。分离在CarboPac PA200柱（250 mm×3 mm）上进行，以NaOH-NaOAc为流动相进行梯度洗脱。校准曲线表明，低聚木糖在0.804-8.607 mg/L范围内具有良好的线性。检测限（LOD）和定量限（LOQ）分别为0.064-0.111 mg/L和0.214-0.371 mg/L。在优化条件下，低聚木糖在三个不同加标水平下的回收率为84.29%-118.19%，相对标准偏差（RSD，n = 3）为0.44%-14.87%。该方法用于低聚木糖产品的定量分析快速且准确。
