Li Jianwen, Wang Zhu, Yang Yuexin
Institute for Nutrition and Food Safety, Chinese CDC, Beijing 100050, China.
Wei Sheng Yan Jiu. 2008 May;37(3):359-61, 371.
A methods for the simultaneous determination for 1-kestose (GF2) and nystose (GF3) in foods and vegetables by high performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-PAD) was developed in this study.
With a gradient program, and NaOH and sodium acetate as mobile phase, the two fructo-oligosaccharides (FOS) were separated on a CarboPac PA1 column and then detected by a gold electrode.
The linear range were 1.22 - 15.20 microg/g and 1.64 - 18.30 microg/g for GF2 and GF3, and the calculated method detection limit (MDL) were 0.085 microg/g and 0.089 microg/g, respectively. The relative standard deviation of GF2 (6.08 microg/g) and GF3 (7.32 microg/g) were 2.80% and 1.68% (n = 6). The contents of the two fructo- oligosaccharides in chicory root, artichoke and oral liquid were also determined. The results shows chicory root possess the highest GF2 and GF3 contents in all vegetable tissues, 6.61g/100g dw and 6.51g/100g dw, whereas the highest composition of kestose and nystose in food was found in Quantum FOS powder (raw material for additives), 34.13g/100g and 32.12g/100g respectively. The method recoveries of GF2 and GF3 were 93.97% and 97.14%.
This method was sensitive, selective, and simple for FOS quantification in vegetables and food products.
本研究建立了一种采用高效阴离子交换色谱-积分脉冲安培检测法（HPAEC-PAD）同时测定食品和蔬菜中1-蔗果三糖（GF2）和蔗果四糖（GF3）的方法。
采用梯度洗脱程序，以氢氧化钠和醋酸钠为流动相，在CarboPac PA1柱上分离两种低聚果糖（FOS），然后用金电极进行检测。
GF2和GF3的线性范围分别为1.22 - 15.20μg/g和1.64 - 18.30μg/g，计算得到的方法检出限（MDL）分别为0.085μg/g和0.089μg/g。GF2（6.08μg/g）和GF3（7.32μg/g）的相对标准偏差分别为2.80%和1.68%（n = 6）。还测定了菊苣根、洋蓟和口服液中两种低聚果糖的含量。结果表明，菊苣根在所有蔬菜组织中GF2和GF3含量最高，分别为6.61g/100g干重和6.51g/100g干重，而食品中蔗果三糖和蔗果四糖含量最高的是量子FOS粉（添加剂原料），分别为34.13g/10百克和32.12g/100g。GF2和GF3的方法回收率分别为93.97%和97.14%。
该方法用于蔬菜和食品中低聚果糖的定量分析灵敏、选择性好且操作简便。
