Department of Neurology, College of Medicine and Neuroscience Research Institute, Medical Research Center, Seoul National University College of Medicine, Seoul, South Korea.
Int J Neurosci. 2011 Aug;121(8):457-61. doi: 10.3109/00207454.2011.576792. Epub 2011 May 17.
We investigated the functional and histological recovery of middle cerebral artery occluded (MCAo) rats after of duplicate intravenous (i.v.) injection of human neural stem cells (hNSCs). Rats received i.v. injections of hNSCs (HB1.F3, 4 × 10(6) cells) on day 1 (1C), day 7 (7C), or both days 1 and 7 (1/7C) following MCAo. Functional recovery of rats was evaluated 1 day before MCAo and 1, 7, 14, 21, and 28 days following MCAo, using the modified neurological severity score (mNSS), and cylinder test. Nissl staining and anti-human nuclear matrix antigen /NeuN or GFAP were used to measure infarct size and investigate the migration and differentiation of injected cells. Treatment with hNSCs did not significantly affect infarct size of ischemic animals. Behavior evaluation using mNSS showed that functional deficits in the 1C group were reduced faster than in the 7C and 1/7C groups, and functional recovery in 1/7C rats was significantly more pronounced than that in the 7C group (day 21). Injected cells were identified at the boundary of lesions, where they had differentiated into neurons and astrocytes. Our study suggests that duplicate i.v. administration of hNSCs after stroke offers no advantages over single administration, 1 day following an ischemic event.
我们研究了重复静脉注射（i.v.）人神经干细胞（hNSCs）后，大脑中动脉闭塞（MCAo）大鼠的功能和组织学恢复情况。大鼠在 MCAo 后第 1 天（1C）、第 7 天（7C）或第 1 天和第 7 天（1/7C）接受 hNSCs（HB1.F3，4×10^6 个细胞）静脉注射。在 MCAo 前 1 天、MCAo 后第 1、7、14、21 和 28 天，通过改良神经功能缺损评分（mNSS）和圆筒试验评估大鼠的功能恢复情况。用尼氏染色和抗人核基质抗原/NeuN 或 GFAP 测量梗死面积，并研究注射细胞的迁移和分化情况。hNSCs 治疗对缺血动物的梗死面积没有显著影响。使用 mNSS 的行为评估表明，1C 组的功能缺陷恢复速度快于 7C 和 1/7C 组，而 1/7C 组的功能恢复明显优于 7C 组（第 21 天）。注射的细胞在病变边界处被鉴定出来，在那里它们已经分化为神经元和星形胶质细胞。我们的研究表明，与缺血事件后第 1 天单次给予 hNSCs 相比，脑卒中后重复静脉给予 hNSCs 没有优势。
