Qiu Dong, Wang Wen-Ling, Zhou Jian-Jiang
Department of Abdominal Oncology, Cancer Hospital of Guizhou Province, Guiyang 550004, China.
Zhonghua Zhong Liu Za Zhi. 2011 Jan;33(1):32-6.
By quantitative detection of telomerase expression, we investigated the relationship between telomerase expression and malignant behavior and prognosis in gastric carcinoma.
A real-time quantitative RT-PCR (RQ-PCR) was used to quantify the hTERT mRNA copy numbers in 89 samples of gastric carcinoma and corresponding non-cancerous tissues. The clinicopathological data of enrolled patients such as age, sex, tumor size, tumor site, pathologic type, histodifferentiation, infiltration depth, lymph node metastasis, stage and survival were obtained, and were made one factor analysis of variance and COX regression prognostic analysis with those above mentioned markers. Follow-up was completed as of February 28, 2010. The median follow-up was 24 months.
hTERT from gastric carcinomas and corresponding non-cancerous tissues was 16.98 ± 3.56 and 11.37 ± 2.15, respectively (P < 0.05), the telomerase activity in gastric cancer was significantly higher than that in non-cancerous tissue (P < 0.05). Telomerase activity showed a positive correlation with depth of invasion, tumor differentiation and nodal metastasis (P < 0.01), and negative correlation with survival.
Gastric cancer with high hTERT mRNA expression indicates a more malignant potential. Detection of hTERT mRNA in gastric cancer may be useful in a better understanding of invasion, metastasis, as well as prognosis of gastric cancer and provide a more efficient therapy. The quantitative expression of hTERT mRNA, infiltration depth and pTNM stage are significant afactors affecting the prognosis of patients with gastric carcinoma.
通过定量检测端粒酶表达，研究端粒酶表达与胃癌恶性行为及预后的关系。
采用实时定量逆转录聚合酶链反应（RQ-PCR）对89例胃癌组织及相应癌旁组织中的人端粒酶逆转录酶（hTERT）mRNA拷贝数进行定量分析。收集入选患者的年龄、性别、肿瘤大小、肿瘤部位、病理类型、组织分化程度、浸润深度、淋巴结转移情况、分期及生存等临床病理资料，并与上述指标进行单因素方差分析和COX回归预后分析。随访至2010年2月28日，中位随访时间为24个月。
胃癌组织及相应癌旁组织的hTERT分别为16.98±3.56和11.37±2.15（P<0.05），胃癌中端粒酶活性显著高于癌旁组织（P<0.05）。端粒酶活性与浸润深度、肿瘤分化程度及淋巴结转移呈正相关（P<0.01），与生存呈负相关。
hTERT mRNA高表达的胃癌具有更高的恶性潜能。检测胃癌组织中的hTERT mRNA有助于更好地了解胃癌的侵袭、转移及预后情况，为临床治疗提供更有效的依据。hTERT mRNA定量表达、浸润深度及pTNM分期是影响胃癌患者预后的重要因素。
