Nachbaur D, Denz H, Zwierzina H, Schmalzl F, Huber H
Department of Haematology-Oncology, University Hospital of Internal Medicine, Innsbruck, Austria.
Cancer Lett. 1990 Apr 30;50(3):197-201. doi: 10.1016/0304-3835(90)90265-y.
We studied the effects human recombinant granulocyte-macrophage colony-stimulating factor and human recombinant interleukin-3 on the colony formation of three human solid tumor cell lines. Using a modified double-layer soft agar clonogenic assay rhGM-CSF enhanced colony formation of all cell lines tested in a dose dependent manner (up to twofold for the breast cancer cell line BT-20, up to 163% of the control for the hypernephroma cell line C 94 and up to 147% for the non-small cell lung cancer cell line CCL 185 at a concentration of 100 ng/ml). RhIL-3 stimulated colony formation of the cell lines C 94 and BT-20, whereas on the cell line CCL 185 rhIL-3 had no effect even at the highest dose level tested (100 ng/ml). Combinations of growth factors showed subadditive stimulation on two cell lines tested (BT-20, C 94). These data indicate that haematopoietic growth factors exert a growth promoting activity on certain solid tumor cells in vitro at physiological concentrations. Therefore our results suggest that the application of these factors in immuno- and myelosuppressed cancer patients after high dose chemotherapy should be seen in light of a possible co-stimulation of the malignant cells.
我们研究了人重组粒细胞-巨噬细胞集落刺激因子和人重组白细胞介素-3 对三种人实体瘤细胞系集落形成的影响。采用改良的双层软琼脂克隆形成试验，rhGM-CSF 以剂量依赖方式增强了所有受试细胞系的集落形成（对于乳腺癌细胞系 BT-20 高达两倍，对于肾细胞癌细胞系 C 94 在浓度为 100 ng/ml 时高达对照的 163%，对于非小细胞肺癌细胞系 CCL 185 高达 147%）。RhIL-3 刺激了细胞系 C 94 和 BT-20 的集落形成，而对于细胞系 CCL 185，即使在测试的最高剂量水平（100 ng/ml），rhIL-3 也没有作用。生长因子组合对两种受试细胞系（BT-20、C 94）显示出亚相加刺激作用。这些数据表明，造血生长因子在生理浓度下对某些实体瘤细胞在体外发挥生长促进活性。因此，我们的结果表明，在高剂量化疗后的免疫抑制和骨髓抑制癌症患者中应用这些因子时，应考虑到对恶性细胞可能的协同刺激作用。
