由具有12q13----q15重排的人良性涎腺肿瘤细胞的SV40“早期区域”进行的体外转化。
In vitro transformation by the SV40 'early region' of cells from a human benign salivary gland tumor with a 12q13----q15 rearrangement.
作者信息
Kazmierczak B, Bartnitzke S, Hartl M, Bullerdiek J
机构信息
Center of Human Genetics and Genetic Counselling, University of Bremen, FRG.
出版信息
Cytogenet Cell Genet. 1990;53(1):37-9. doi: 10.1159/000132891.
Cells from a salivary gland pleomorphic adenoma with a reciprocal translocation, t(8;12)(q22;q14), were transformed by transfection with a plasmid containing the SV40 "early region." Compared with nontransformed adenoma cells, the cells expressed large-T antigen, had a higher proliferative capacity, showed a drastically increased or even unlimited growth potential in vitro, and formed colonies in soft agar. The amount of cellular material produced in this way should make possible many experiments at the molecular level that could not be performed before.
来自唾液腺多形性腺瘤且带有相互易位t(8;12)(q22;q14)的细胞，通过用含有SV40“早期区域”的质粒转染而被转化。与未转化的腺癌细胞相比，这些细胞表达大T抗原，具有更高的增殖能力，在体外显示出急剧增加甚至无限的生长潜力，并能在软琼脂中形成集落。以这种方式产生的细胞物质数量应使许多以前无法进行的分子水平实验成为可能。
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