Department of Neurobiology, University of Alabama Birmingham, Birmingham, AL 35294-0021, United States.
Schizophr Res. 2011 Aug;130(1-3):260-5. doi: 10.1016/j.schres.2011.04.029. Epub 2011 May 14.
Several lines of evidence indicate altered trafficking of α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptors in schizophrenia. Previous reports have implicated alterations in the endosomal trafficking of AMPA receptors in this illness. We hypothesized that late endosome content of AMPA receptor subunits is altered in schizophrenia. Accordingly, we developed a technique to isolate and measure contents of late endosomes from postmortem human tissue. We found no changes in the expression of the AMPA subunits, GluR1-4, in late endosomes from the dorsolateral prefrontal cortex in schizophrenia. We also hypothesized that proteins involved in the sorting and trafficking of AMPA receptors between endosomal compartments would be altered in schizophrenia. We found no changes in expression of multiple proteins associated with these processes (dynamin3, Arc/ARG3.1, NEEP21, GRASP1, liprin α, and syntaxin13). Together, these data suggest that endosomal trafficking of AMPA receptors in the prefrontal cortex may be largely intact in schizophrenia.
有几条证据表明，精神分裂症患者的α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体的转运发生了改变。先前的报告表明，AMPA 受体的内体转运在这种疾病中发生了改变。我们假设，精神分裂症患者的晚期内体中 AMPA 受体亚基的含量发生了改变。因此，我们开发了一种从人死后组织中分离和测量晚期内体含量的技术。我们发现在精神分裂症患者的背外侧前额叶皮层的晚期内体中，AMPA 亚基 GluR1-4 的表达没有变化。我们还假设，参与 AMPA 受体在内体隔室之间分拣和运输的蛋白质在精神分裂症中会发生改变。我们发现与这些过程相关的多种蛋白质的表达没有变化（dynamin3、Arc/ARG3.1、NEEP21、GRASP1、liprin α 和 syntaxin13）。综上所述，这些数据表明，精神分裂症患者前额叶皮层中的 AMPA 受体的内体转运可能基本完整。
