Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu 300, Taiwan, ROC.
In Vivo. 2011 May-Jun;25(3):393-8.
Danthron has been shown to induce apoptotic cell death, and inhibit migration and invasion of human gastric or brain cancer cells in vitro. However, there is no report addressing whether danthron affects murine leukemia cells or immune responses in vivo. Herein, this study focused on the in-vivo effects of danthron on WEHI-3 leukemia in mice and immune responses in vivo. The results indicated that danthron reduced spleen weight and increased the percentage of cells with CD3 and CD19 markers, indicating that differentiation of the precursors of T- and B-cells was promoted in the leukemic mice. The results also showed that danthron promoted the activity of phagocytosis by macrophages isolated from the peritoneal cavity but had no effect on peripheral blood mononuclear cells. Danthron also promoted natural killer cell cytocytic activity at an effector and target cell ratio of 100:1 in comparison with leukemic animals in vivo. Taken together, these results demonstrated that application of danthron might affect WEHI-3 leukemia in mice and modulate immune responses in vivo.
蒽酮已被证明能诱导细胞凋亡，并能抑制体外培养的人胃癌或脑癌细胞的迁移和侵袭。然而，目前尚无报道表明蒽酮是否影响体内的小鼠白血病细胞或免疫反应。在此，本研究集中于蒽酮对 WEHI-3 白血病小鼠体内作用和体内免疫反应的影响。结果表明，蒽酮降低了脾脏重量，并增加了具有 CD3 和 CD19 标志物的细胞的百分比，表明白血病小鼠中的 T 细胞和 B 细胞前体的分化得到了促进。结果还表明，蒽酮促进了腹腔分离的巨噬细胞的吞噬作用活性，但对外周血单个核细胞没有影响。与体内白血病动物相比，蒽酮在效应细胞与靶细胞比例为 100:1 时也促进了自然杀伤细胞的细胞毒性活性。综上所述，这些结果表明，蒽酮的应用可能会影响 WEHI-3 白血病小鼠，并调节体内免疫反应。
