Department of Pharmacology, China Medical University, No. 91 Hsueh-Shih Road, Taichung, Taiwan, ROC.
Hum Exp Toxicol. 2010 May;29(5):359-67. doi: 10.1177/0960327110364153. Epub 2010 Mar 2.
Solanum lyratum Thunberg (Solanaceae) has been used as a folk medicine for treating liver, lung and esophagus in the Chinese population. Our previous studies have shown that the crude extract of S. lyratum Thunberg (SLE) induced apoptosis in colo 205 human colon adenocarcinoma cells; however, there is no report to show SLE affect immune responses in vivo. In this study, the in vivo effects of SLE on leukemia WEHI-3 cells and immune responses such as phagocytosis and natural killer (NK) cell activity in normal and leukemia mice were investigated. The SLE treatment decreases surface markers of CD3 and Mac-3 in normal and leukemia mice but promoted the cell markers of CD19 and CD11b in normal mice and CD11b in leukemia mice indicating that the precursors of T cells was inhibited and B cells and macrophage were promoted. The SLE treatment promoted the activity of macrophage phagocytosis in the peripheral blood mononuclear cells (PBMC) and peritoneal cells from normal and leukemia mice. The results also showed that NK cells from the normal and leukemia mice after treatment with SLE can kill the YAC-1 target cells. Therefore, the SLE treatment increased macrophage and NK cell activities. These consistent results indicate SLE could be a potent immune responses agent.
莨菪（茄科）已被民间用作治疗肝脏、肺部和食管疾病的药物。我们之前的研究表明，莨菪的粗提物（SLE）可诱导结肠 205 人结肠腺癌细胞凋亡；然而，目前尚无报道表明 SLE 会影响体内的免疫反应。在这项研究中，我们研究了 SLE 对白血病 WEHI-3 细胞以及正常和白血病小鼠体内吞噬作用和自然杀伤（NK）细胞活性等免疫反应的体内影响。SLE 治疗可降低正常和白血病小鼠中 CD3 和 Mac-3 的表面标志物，但可促进正常小鼠的 CD19 和 CD11b 以及白血病小鼠的 CD11b 细胞标志物，表明 T 细胞前体受到抑制，B 细胞和巨噬细胞受到促进。SLE 治疗可促进正常和白血病小鼠外周血单核细胞（PBMC）和腹腔细胞中巨噬细胞的吞噬作用。结果还表明，SLE 处理后正常和白血病小鼠的 NK 细胞可杀死 YAC-1 靶细胞。因此，SLE 治疗可增强巨噬细胞和 NK 细胞的活性。这些一致的结果表明，SLE 可能是一种有效的免疫反应调节剂。
