bla(KPC) RNA 表达与两个转录起始位点相关，但并不总是与四种革兰氏阴性病原体的基因拷贝数相关。
bla(KPC) RNA expression correlates with two transcriptional start sites but not always with gene copy number in four genera of Gram-negative pathogens.
机构信息
Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, NE 68178, USA.
出版信息
Antimicrob Agents Chemother. 2011 Aug;55(8):3936-8. doi: 10.1128/AAC.01509-10. Epub 2011 May 16.
Abstract
Klebsiella pneumoniae carbapenemase (KPC)-producing organisms are therapeutically and diagnostically challenging. It is possible that bla(KPC) gene expression plays a role in the variability observed in clinical susceptibility testing. bla(KPC) transformants together with 10 clinical isolates representing four genera were evaluated for bla(KPC) copy number and gene expression and correlated with β-lactam MIC data. The data suggest that mechanisms other than gene copy number and expression of bla(KPC) contribute to variability in susceptibility when testing KPC-producing isolates.
摘要
产碳青霉烯酶肺炎克雷伯菌（KPC）的治疗和诊断极具挑战性。bla（KPC）基因表达可能在临床药敏试验中观察到的变异性中起作用。评估了bla（KPC）转化体以及代表四个属的 10 株临床分离株的 bla（KPC）拷贝数和基因表达，并与β-内酰胺 MIC 数据相关联。数据表明，当测试产 KPC 分离株时，bla（KPC）基因拷贝数和表达以外的机制有助于变异性。
相似文献
1
bla(KPC) RNA expression correlates with two transcriptional start sites but not always with gene copy number in four genera of Gram-negative pathogens.bla(KPC) RNA 表达与两个转录起始位点相关，但并不总是与四种革兰氏阴性病原体的基因拷贝数相关。
Antimicrob Agents Chemother. 2011 Aug;55(8):3936-8. doi: 10.1128/AAC.01509-10. Epub 2011 May 16.
2
Phenotypic and molecular detection of the bla gene in clinical isolates from inpatients at hospitals in São Luis, MA, Brazil.巴西马塞约州圣路易斯市医院住院患者临床分离株中bla基因的表型和分子检测
BMC Infect Dis. 2016 Dec 7;16(1):737. doi: 10.1186/s12879-016-2072-3.
3
Emergence of clonally related Klebsiella pneumoniae isolates of sequence type 258 producing plasmid-mediated KPC carbapenemase in Norway and Sweden.挪威和瑞典出现序列型258的克隆相关肺炎克雷伯菌分离株，这些分离株产生质粒介导的KPC碳青霉烯酶。
J Antimicrob Chemother. 2009 Apr;63(4):654-8. doi: 10.1093/jac/dkp018. Epub 2009 Feb 13.
4
Promoter deletions of Klebsiella pneumoniae carbapenemase (KPC)-encoding genes (blaKPC -2) and efflux pump (AcrAB) on β-lactam susceptibility in KPC-producing Enterobacteriaceae.肺炎克雷伯菌碳青霉烯酶（KPC）编码基因（blaKPC-2）和外排泵（AcrAB）在产 KPC 肠杆菌科细菌对β-内酰胺类药物敏感性中的启动子缺失。
FEMS Microbiol Lett. 2013 Nov;348(2):120-6. doi: 10.1111/1574-6968.12273. Epub 2013 Sep 30.
5
Emergence of bla KPC-3-Tn4401a associated with a pKPN3/4-like plasmid within ST384 and ST388 Klebsiella pneumoniae clones in Spain.西班牙产 ST384 和 ST388 肺炎克雷伯菌克隆中出现的 bla KPC-3-Tn4401a 与 pKPN3/4 样质粒相关。
J Antimicrob Chemother. 2010 Aug;65(8):1608-14. doi: 10.1093/jac/dkq174. Epub 2010 May 30.
6
Phenotypic and genotypic screening and clonal analysis of carbapenem-resistant Klebsiella pneumoniae at a single hospital.单家医院碳青霉烯类耐药肺炎克雷伯菌的表型和基因型筛查及克隆分析。
Microb Drug Resist. 2011 Jun;17(2):251-7. doi: 10.1089/mdr.2010.0116. Epub 2011 Feb 19.
7
Phenotypic and genotypic characterization of β-lactam resistance in Klebsiella pneumoniae isolated from swine.猪源肺炎克雷伯菌β-内酰胺类耐药表型及基因型特征。
Vet Microbiol. 2011 Apr 21;149(1-2):139-46. doi: 10.1016/j.vetmic.2010.09.030. Epub 2010 Oct 7.
8
Arrival of Klebsiella pneumoniae producing KPC carbapenemase in the United Kingdom.产KPC碳青霉烯酶肺炎克雷伯菌在英国出现。
J Antimicrob Chemother. 2008 Dec;62(6):1261-4. doi: 10.1093/jac/dkn396. Epub 2008 Sep 23.
9
Emergence of carbapenemase-producing Gram-negative bacteria in Saint Petersburg, Russia.俄罗斯圣彼得堡产碳青霉烯酶革兰阴性菌的出现。
Int J Antimicrob Agents. 2014 Aug;44(2):152-5. doi: 10.1016/j.ijantimicag.2014.05.004. Epub 2014 Jun 12.
10
Characterization of blaKPC-containing Klebsiella pneumoniae isolates detected in different institutions in the Eastern USA.在美国东部不同机构检测到的携带blaKPC的肺炎克雷伯菌分离株的特征分析。
J Antimicrob Chemother. 2009 Mar;63(3):427-37. doi: 10.1093/jac/dkn547. Epub 2009 Jan 20.
引用本文的文献
1
Large-scale analysis of putative plasmids in clinical multidrug-resistant isolates from Vietnamese patients.对越南患者临床多重耐药分离株中假定质粒的大规模分析。
Front Microbiol. 2023 May 31;14:1094119. doi: 10.3389/fmicb.2023.1094119. eCollection 2023.
2
Prevalence of carbapenemase genes among carbapenem-nonsusceptible collected in US hospitals in a five-year period and activity of ceftazidime/avibactam and comparator agents.五年期间在美国医院收集的碳青霉烯不敏感菌中碳青霉烯酶基因的流行情况以及头孢他啶/阿维巴坦和对照药物的活性。
JAC Antimicrob Resist. 2022 Sep 30;4(5):dlac098. doi: 10.1093/jacamr/dlac098. eCollection 2022 Oct.
3
Genomic Features Associated with the Degree of Phenotypic Resistance to Carbapenems in Carbapenem-Resistant Klebsiella pneumoniae.耐碳青霉烯类肺炎克雷伯菌中与碳青霉烯类药物表型耐药程度相关的基因组特征
mSystems. 2021 Oct 26;6(5):e0019421. doi: 10.1128/mSystems.00194-21. Epub 2021 Sep 14.
4
Antimicrobial Resistance Caused by KPC-2 Encoded by Promiscuous Plasmids of the Klebsiella pneumoniae ST307 Strain.由肺炎克雷伯菌 ST307 株的多功能质粒编码的 KPC-2 引起的抗生素耐药性。
Ann Lab Med. 2021 Jan;41(1):86-94. doi: 10.3343/alm.2021.41.1.86. Epub 2020 Aug 25.
5
Reduced Ceftazidime-Avibactam Susceptibility in KPC-Producing From Patients Without Ceftazidime-Avibactam Use History - A Multicenter Study in China.中国一项多中心研究：既往无头孢他啶-阿维巴坦使用史的产KPC菌株对头孢他啶-阿维巴坦的敏感性降低
Front Microbiol. 2020 Jun 23;11:1365. doi: 10.3389/fmicb.2020.01365. eCollection 2020.
6
High-performance method to detection of Klebsiella pneumoniae Carbapenemase in Enterobacterales by LC-MS/MS.LC-MS/MS 法高效检测肠杆菌科中的肺炎克雷伯菌碳青霉烯酶。
Braz J Microbiol. 2020 Sep;51(3):1029-1035. doi: 10.1007/s42770-019-00222-y. Epub 2020 Jan 27.
7
Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens.铜绿假单胞菌 - 改良霍奇试验（PAE-MHT）及ChromID Carba琼脂用于检测从临床标本中分离出的产碳青霉烯酶铜绿假单胞菌
Open Access Maced J Med Sci. 2018 Dec 15;6(12):2283-2289. doi: 10.3889/oamjms.2018.414. eCollection 2018 Dec 20.
8
Transcriptional Landscape of a Plasmid and Response to Imipenem Exposure in TOP10.TOP10中质粒的转录图谱及对亚胺培南暴露的反应
Front Microbiol. 2018 Dec 3;9:2929. doi: 10.3389/fmicb.2018.02929. eCollection 2018.
9
IS-mediated transposition of chromosome-borne into an endogenous ColE1-like plasmid in .在……中，插入序列介导的染色体携带片段转座到内源性ColE1样质粒中。
Infect Drug Resist. 2018 Jul 23;11:995-1005. doi: 10.2147/IDR.S159345. eCollection 2018.
10
Enhanced Klebsiella pneumoniae Carbapenemase Expression from a Novel Tn Deletion.新型Tn缺失导致肺炎克雷伯菌碳青霉烯酶表达增强
Antimicrob Agents Chemother. 2017 May 24;61(6). doi: 10.1128/AAC.00025-17. Print 2017 Jun.
本文引用的文献
1
Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI.根瘤菌属豌豆根瘤 Sym 质粒 pRL1JI 结瘤区的启动子。
Plant Mol Biol. 1987 Jan;9(1):27-39. doi: 10.1007/BF00017984.
2
Genetic factors associated with elevated carbapenem resistance in KPC-producing Klebsiella pneumoniae.与产 KPC 肺炎克雷伯菌碳青霉烯类耐药升高相关的遗传因素。
Antimicrob Agents Chemother. 2010 Oct;54(10):4201-7. doi: 10.1128/AAC.00008-10. Epub 2010 Jul 26.
3
Contribution of OmpK36 to carbapenem susceptibility in KPC-producing Klebsiella pneumoniae.外膜孔蛋白K36对产KPC肺炎克雷伯菌碳青霉烯类药物敏感性的影响
J Med Microbiol. 2009 Oct;58(Pt 10):1303-1308. doi: 10.1099/jmm.0.012575-0. Epub 2009 Jun 25.
4
Genetic organization of transposase regions surrounding blaKPC carbapenemase genes on plasmids from Klebsiella strains isolated in a New York City hospital.从纽约市一家医院分离出的克雷伯氏菌菌株质粒上 blaKPC 碳青霉烯酶基因周围转座酶区域的基因组织。
Antimicrob Agents Chemother. 2009 May;53(5):1998-2004. doi: 10.1128/AAC.01355-08. Epub 2009 Mar 2.
5
Characterization of blaKPC-containing Klebsiella pneumoniae isolates detected in different institutions in the Eastern USA.在美国东部不同机构检测到的携带blaKPC的肺炎克雷伯菌分离株的特征分析。
J Antimicrob Chemother. 2009 Mar;63(3):427-37. doi: 10.1093/jac/dkn547. Epub 2009 Jan 20.
6
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.新型KPC变体KPC-5及其进化变体KPC-2和KPC-4的表型和酶学比较分析。
Antimicrob Agents Chemother. 2009 Feb;53(2):557-62. doi: 10.1128/AAC.00734-08. Epub 2008 Nov 17.
7
Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes.利用定量实时 PCR 提高质粒拷贝数的测定，用于监测发酵过程。
Microb Cell Fact. 2008 Mar 7;7:6. doi: 10.1186/1475-2859-7-6.
8
High-level carbapenem resistance in a Citrobacter freundii clinical isolate is due to a combination of KPC-2 production and decreased porin expression.弗氏柠檬酸杆菌临床分离株中的高水平碳青霉烯耐药性是由KPC-2产生和孔蛋白表达降低共同导致的。
J Med Microbiol. 2008 Mar;57(Pt 3):332-337. doi: 10.1099/jmm.0.47576-0.
9
Carbapenem resistance in Klebsiella pneumoniae not detected by automated susceptibility testing.自动药敏试验未检测到肺炎克雷伯菌对碳青霉烯类药物的耐药性。
Emerg Infect Dis. 2006 Aug;12(8):1209-13. doi: 10.3201/eid1208.060291.
10
Plasmid-mediated imipenem-hydrolyzing enzyme KPC-2 among multiple carbapenem-resistant Escherichia coli clones in Israel.以色列多种耐碳青霉烯类大肠杆菌克隆株中质粒介导的碳青霉烯水解酶KPC-2
Antimicrob Agents Chemother. 2006 Sep;50(9):3098-101. doi: 10.1128/AAC.00438-06.
Zotero 插件