Glycotechnology Business Unit, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438–0802, Japan.
Microbes Environ. 2010;25(3):152-5. doi: 10.1264/jsme2.me10118.
Oligosaccharides containing N-acetylneuraminic acid on the cell surface of some pathogenic bacteria are important for host-microbe interactions. N-acetylneuraminic acid (Neu5Ac) plays a major role in the pathogenicity of bacterial pathogens. For example, cell surface sialyloligosaccharide moieties of the human pathogen Haemophilus influenzae are involved in virulence and adhesion to host cells. In this study, we have established a method of visualizing Neu5Ac linked to a glycoconjugate on the bacterial cell surface based on lectin staining. Photobacterium damselae strain JT0160, known to produce a-2,6-sialyltransferase, was revealed to possess Neu5Ac by HPLC. Using the strain, a strong Sambucus sieboldiana lectin-binding signal was detected. The bacteria producing α-2,6-sialyltransferases could be divided into two groups: those with a lot of α-2,6-linked Neu5Ac on the cell surface and those with a little. In the present study, we developed a useful method for evaluating the relationship between Neu5Ac expression on the cell surface and the degree of virulence of marine bacteria.
某些致病菌细胞表面含 N-乙酰神经氨酸的寡糖在宿主-微生物相互作用中起着重要作用。N-乙酰神经氨酸（Neu5Ac）在细菌病原体的致病性中起主要作用。例如，人类病原体流感嗜血杆菌细胞表面的唾液酸化寡糖部分参与了毒力和与宿主细胞的黏附。在这项研究中，我们建立了一种基于凝集素染色可视化与糖缀合物相连的 Neu5Ac 的方法。已知产生 a-2,6-唾液酸转移酶的 Photobacterium damselae 菌株 JT0160 通过 HPLC 被揭示含有 Neu5Ac。使用该菌株，检测到与 Sambucus sieboldiana 凝集素强烈结合的信号。产生α-2,6-唾液酸转移酶的细菌可以分为两组：一组在细胞表面有大量的α-2,6 连接的 Neu5Ac，另一组则较少。在本研究中，我们开发了一种评估海洋细菌表面 Neu5Ac 表达与毒力程度之间关系的有用方法。
