Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan.
Microbes Environ. 2010;25(3):183-9. doi: 10.1264/jsme2.me10114.
We examined the abundance and community structure of sphingomonads in the decaying leaf residues of eight plant species as well as the nearby soil, by 16S rRNA gene-based real-time PCR and denaturing gradient gel electrophoresis. In the leaf residues, the sphingomonads generally accumulated to high levels, comprising approximately 15% of the total bacteria, and formed a community structure related to sampling locations. At least within the time period studied, their abundance in leaf residues changed, but their community structure was basically maintained. In soil, sphingomonads made up only 1.7% of total bacteria on average. The community structure of sphingomonads differed between the leaf residues and bulk soil, among plant plots, and among samples collected at different times. The results show that particular sphingomonad populations accumulate in leaf residues compared to the surrounding bulk soil under field conditions.
我们通过基于 16S rRNA 基因的实时 PCR 和变性梯度凝胶电泳技术，研究了 8 种植物凋落叶残渣及其附近土壤中鞘氨醇单胞菌的丰度和群落结构。在叶残渣中，鞘氨醇单胞菌通常积累到很高的水平，占总细菌的约 15%，并形成与采样地点相关的群落结构。至少在研究期间内，它们在叶残渣中的丰度发生了变化，但群落结构基本保持不变。在土壤中，鞘氨醇单胞菌平均仅占总细菌的 1.7%。鞘氨醇单胞菌的群落结构在叶残渣和原状土壤之间、不同植物样地之间以及不同时间采集的样本之间存在差异。结果表明，与周围原状土壤相比，特定的鞘氨醇单胞菌种群在田间条件下会在叶残渣中积累。
