Jin Hui, Yang Xiao-Yan, Yan Zhi-Qiang, Liu Quan, Li Xiu-Zhuang, Chen Ji-Xiang, Zhang Deng-Hong, Zeng Li-Ming, Qin Bo
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
Syst Appl Microbiol. 2014 Jul;37(5):376-85. doi: 10.1016/j.syapm.2014.05.001. Epub 2014 May 20.
A diverse array of bacteria that inhabit the rhizosphere and different plant organs play a crucial role in plant health and growth. Therefore, a general understanding of these bacterial communities and their diversity is necessary. Using the 16S rRNA gene clone library technique, the bacterial community structure and diversity of the rhizosphere and endophytic bacteria in Stellera chamaejasme compartments were compared and clarified for the first time. Grouping of the sequences obtained showed that members of the Proteobacteria (43.2%), Firmicutes (36.5%) and Actinobacteria (14.1%) were dominant in both samples. Other groups that were consistently found, albeit at lower abundance, were Bacteroidetes (2.1%), Chloroflexi (1.9%), and Cyanobacteria (1.7%). The habitats (rhizosphere vs endophytes) and organs (leaf, stem and root) structured the community, since the Wilcoxon signed rank test indicated that more varied bacteria inhabited the rhizosphere compared to the organs of the plant. In addition, correspondence analysis also showed that differences were apparent in the bacterial communities associated with these distinct habitats. Moreover, principal component analysis revealed that the profiles obtained from the rhizosphere and roots were similar, whereas leaf and stem samples clustered together on the opposite side of the plot from the rhizosphere and roots. Taken together, these results suggested that, although the communities associated with the rhizosphere and organs shared some bacterial species, the associated communities differed in structure and diversity.
大量栖息于根际和不同植物器官的细菌对植物健康和生长起着至关重要的作用。因此，全面了解这些细菌群落及其多样性很有必要。利用16S rRNA基因克隆文库技术，首次对瑞香狼毒各部位根际和内生细菌的群落结构及多样性进行了比较和阐明。对所得序列进行分组显示，变形菌门（43.2%）、厚壁菌门（36.5%）和放线菌门（14.1%）的成员在两个样本中均占主导地位。其他始终被发现的类群，尽管丰度较低，包括拟杆菌门（2.1%）、绿弯菌门（1.9%）和蓝细菌（1.7%）。生境（根际与内生菌）和器官（叶、茎和根）构建了群落结构，因为威尔科克森符号秩检验表明，与植物器官相比，根际栖息着更多种类的细菌。此外，对应分析还表明，与这些不同生境相关的细菌群落存在明显差异。而且，主成分分析显示，从根际和根获得的图谱相似，而叶和茎样本在图谱的另一侧聚集在一起，与根际和根相对。综上所述，这些结果表明，尽管与根际和器官相关的群落共享一些细菌物种，但相关群落的结构和多样性存在差异。
