Department of Marine Bioscience, Fukui Prefectural University, Obama, Fukui 917–0003, Japan.
Microbes Environ. 2010;25(3):190-6. doi: 10.1264/jsme2.me10109.
A new set of primers for the detection of phototrophic sulfur bacteria in natural environments is described. The primers target the α-subunit of the reverse dissimilatory sulfite reductase gene (dsrA). PCR-amplification resulted in products of the expected size from all the phototrophic strains tested, including purple sulfur and green sulfur bacteria. Seventy-nine clones obtained from environmental DNA using the primers were sequenced and all found to be closely related to the dsrA of purple sulfur bacteria and green sulfur bacteria. This newly developed PCR assay targeting dsrA is rapid and simple for the detection of phototrophic sulfur bacteria in situ and superior to the use of culture-dependent techniques.
本文介绍了一种新的用于检测自然环境中光能自养硫细菌的引物。这些引物针对的是反向异化亚硫酸盐还原酶基因（dsrA）的α亚基。PCR 扩增从所有测试的光能自养菌中得到了预期大小的产物，包括紫硫菌和绿硫菌。用引物从环境 DNA 中获得的 79 个克隆进行了测序，结果均与紫硫菌和绿硫菌的 dsrA 密切相关。这种新开发的针对 dsrA 的 PCR 检测方法快速、简单，可用于原位检测光能自养硫细菌，优于使用依赖培养的技术。
