Roeselers Guus, Norris Tracy B, Castenholz Richard W, Rysgaard Søren, Glud Ronnie N, Kühl Michael, Muyzer Gerard
Department of Biotechnology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, the Netherlands.
Environ Microbiol. 2007 Jan;9(1):26-38. doi: 10.1111/j.1462-2920.2006.01103.x.
We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail before. Specific oligonucleotide primers for cyanobacteria, purple sulfur bacteria, green sulfur bacteria and Choroflexus/Roseiflexus-like green non-sulfur bacteria were used for the selective amplification of 16S rRNA gene fragments. Amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced. In addition, several cyanobacteria were isolated from the mat samples, and classified morphologically and by 16S rRNA-based methods. The cyanobacterial 16S rRNA sequences obtained from DGGE represented a diverse, polyphyletic collection of cyanobacteria. The microbial mat communities were dominated by heterocystous and non-heterocystous filamentous cyanobacteria. Our results indicate that the cyanobacterial community composition in the samples were different for each sampling site. Different layers of the same heterogeneous mat often contained distinct and different communities of cyanobacteria. We observed a relationship between the cyanobacterial community composition and the in situ temperatures of different mat parts. The Greenland mats exhibited a low diversity of anoxygenic phototrophs as compared with other hot spring mats which is possibly related to the photochemical conditions within the mats resulting from the Arctic light regime.
我们对从格陵兰岛东海岸三个温泉地点采集的微生物席样本中的产氧和不产氧光合微生物的基因型多样性进行了调查。这些温泉拥有独特的北极微生物生态系统，此前从未被详细研究过。用于蓝细菌、紫色硫细菌、绿色硫细菌以及类绿弯菌属/玫瑰弯菌属绿色非硫细菌的特异性寡核苷酸引物，被用于选择性扩增16S rRNA基因片段。扩增产物通过变性梯度凝胶电泳（DGGE）进行分离并测序。此外，从微生物席样本中分离出了几种蓝细菌，并通过形态学方法和基于16S rRNA的方法进行了分类。从DGGE获得的蓝细菌16S rRNA序列代表了一个多样的、多系的蓝细菌集合。微生物席群落以具异形胞和不具异形胞的丝状蓝细菌为主。我们的结果表明，每个采样点样本中的蓝细菌群落组成各不相同。同一异质微生物席的不同层通常含有不同且各异的蓝细菌群落。我们观察到蓝细菌群落组成与不同微生物席部分的原位温度之间存在关联。与其他温泉微生物席相比，格陵兰岛的微生物席显示出较低的不产氧光合生物多样性，这可能与北极光照条件导致的微生物席内的光化学条件有关。
