Department of Biology, Niagara University, Lewiston, New York, USA.
Microbiologyopen. 2021 Aug;10(4):e1228. doi: 10.1002/mbo3.1228.
The permanently stratified water columns in euxinic meromictic lakes produce niche environments for phototrophic sulfur oxidizers and diverse sulfur metabolisms. While Green Lake (Fayetteville, New York, NY) is known to host a diverse community of ecologically important sulfur bacteria, analyses of its microbial communities, to date, have been largely based on pigment analysis and smaller datasets from Sanger sequencing techniques. Here, we present the results of next-generation sequencing of the eubacterial community in the context of the water column geochemistry. We observed abundant purple and green sulfur bacteria, as well as anoxygenic photosynthesis-capable cyanobacteria within the upper monimolimnion. Amidst the phototrophs, we found other sulfur-cycling bacteria including sulfur disproportionators and chemotrophic sulfur oxidizers, further detailing our understanding of the sulfur cycle and microbial ecology of euxinic, meromictic lakes.
贫营养分层型湖泊的永久性分层水柱为光能自养硫氧化菌和多样化的硫代谢提供了小生境。虽然众所周知，纽约州费耶特维尔的绿湖（Green Lake）拥有多样化的具有重要生态意义的硫细菌群落，但迄今为止，对其微生物群落的分析主要基于色素分析和桑格测序技术的较小数据集。在这里，我们根据水柱地球化学的情况，介绍了真细菌群落的下一代测序结果。我们在上层的季节性分层湖中观察到了丰富的紫色和绿色硫细菌，以及能够进行厌氧光合作用的蓝细菌。在这些光合生物中，我们还发现了其他参与硫循环的细菌，包括硫歧化菌和化能自养硫氧化菌，这进一步详细说明了我们对贫营养分层型、分层型湖泊的硫循环和微生物生态的理解。
