Department of Biological Sciences, Wright State University, Dayton, Ohio 45435, USA.
Int Arch Allergy Immunol. 2011;156(2):171-8. doi: 10.1159/000323351. Epub 2011 May 17.
Skin contact with house dust mites may contribute to atopic dermatitis and other skin diseases. We sought to determine if molecules from house dust mites could influence the release of proinflammatory cytokines and chemokines from epidermal keratinocytes and dermal fibroblasts grown in a human skin equivalent (HSE) model.
HSEs consisting of an epidermis of keratinocytes with stratum corneum over a dermis of fibroblasts in a collagen matrix were challenged with Dermatophagoides farinae, D. pteronyssinus and Euroglyphus maynei mite extracts.
HSEs secreted interleukin (IL)-1α, IL-1 receptor antagonist, IL-6, IL-8, cutaneous T cell-attracting chemokine, transforming growth factor-α, granulocyte/macrophage and macrophage colony-stimulating factors and vascular endothelial cell growth factor in response to at least 1 mite extract. Extracts of different mite species stimulated HSEs to release different cytokines. Therefore, extracts of different species contained different molecules or different concentrations of similar molecules. The cytokine release profiles of cells in the HSEs were not the same as for monocultured keratinocytes and fibroblasts.
Molecules from house dust mites are capable of inducing the release of multiple proinflammatory cytokines and chemokines from epidermal keratinocytes and dermal fibroblasts. Avoiding skin contact with house dust mites would reduce the possibility of mite-induced inflammation in the skin. Therefore, measures to reduce contact with mite molecules such as frequent vacuuming of upholstered furniture and carpets and laundering of clothing and bedding to remove mite molecules and allergens could reduce skin contact with mite molecules and diminish exacerbations of skin inflammation in patients with atopic dermatitis and other skin diseases.
皮肤接触屋尘螨可能导致特应性皮炎和其他皮肤病。我们试图确定屋尘螨的分子是否可以影响表皮角质形成细胞和真皮成纤维细胞在人皮肤等效物（HSE）模型中释放促炎细胞因子和趋化因子。
HSE 由角质形成细胞的表皮组成，表皮具有角质层，真皮为成纤维细胞，位于胶原基质中。用屋尘螨、粉尘螨和 Euroglyphus maynei 螨提取物对 HSE 进行挑战。
HSE 分泌白细胞介素（IL）-1α、IL-1 受体拮抗剂、IL-6、IL-8、皮肤 T 细胞吸引趋化因子、转化生长因子-α、粒细胞/巨噬细胞和巨噬细胞集落刺激因子和血管内皮细胞生长因子，以响应至少 1 种螨提取物。不同螨种的提取物刺激 HSE 释放不同的细胞因子。因此，不同物种的提取物含有不同的分子或不同浓度的相似分子。HSE 中细胞的细胞因子释放谱与单独培养的角质形成细胞和成纤维细胞不同。
来自屋尘螨的分子能够诱导表皮角质形成细胞和真皮成纤维细胞释放多种促炎细胞因子和趋化因子。避免皮肤与屋尘螨接触将减少皮肤中螨诱导炎症的可能性。因此，减少与螨分子接触的措施，如频繁吸尘有软垫的家具和地毯，以及清洗衣物和床上用品以去除螨分子和过敏原，可以减少皮肤与螨分子的接触，并减轻特应性皮炎和其他皮肤病患者皮肤炎症的恶化。
