London S D, Cebra-Thomas J A, Rubin D H, Cebra J J
Department of Biology, University of Pennsylvania, Philadelphia.
J Immunol. 1990 Apr 15;144(8):3187-94.
CD8 lymphocyte population heterogeneity has been examined by using reovirus serotype 1, strain Lang (reovirus 1/L) as a model infection. We have previously reported that gut mucosal infection with reovirus stimulates the appearance of virus-specific cytotoxic T cell precursors (pCTL) in Peyer's patches (PP). The effectors that mediate reovirus-specific cytotoxicity were found to express both the Thy-1 and CD8 Ag and were MHC-restricted in their recognition of reovirus Ag. To further characterize the virus-specific precursor and effector cells we have analyzed PP cells for the expression of a novel surface Ag (termed germinal center and T cell Ag (GCT)) found on germinal center B cells and a subpopulation of CD8+ T cells. Gut mucosal infection with reovirus 1/L is capable of increasing the proportion of GCT+ CD8+ T cells in PP. Positive selection as well as depletion of GCT+ cells has demonstrated that pCTL can express this Ag, and depletion experiments have demonstrated that effector CTL express the GCT Ag. Thus, a subpopulation of GCT+ cells have been identified as Ag-specific precursor and effector CTL. These observations indicate that the expression of the GCT Ag may provide a means to identify recently stimulated pCTL or effector CTL in gut mucosal tissues.
已使用呼肠孤病毒1型朗株（呼肠孤病毒1/L）作为模型感染来研究CD8淋巴细胞群体的异质性。我们之前报道过，呼肠孤病毒引起的肠道黏膜感染会刺激派伊尔结（PP）中病毒特异性细胞毒性T细胞前体（pCTL）的出现。发现介导呼肠孤病毒特异性细胞毒性的效应细胞同时表达Thy-1和CD8抗原，并且在识别呼肠孤病毒抗原时受主要组织相容性复合体（MHC）限制。为了进一步表征病毒特异性前体细胞和效应细胞，我们分析了PP细胞中一种在生发中心B细胞和CD8+ T细胞亚群上发现的新型表面抗原（称为生发中心和T细胞抗原（GCT））的表达情况。用呼肠孤病毒1/L进行肠道黏膜感染能够增加PP中GCT+ CD8+ T细胞的比例。对GCT+细胞进行阳性选择以及去除实验表明，pCTL可以表达这种抗原，去除实验还表明效应性CTL表达GCT抗原。因此，已将GCT+细胞亚群鉴定为抗原特异性前体细胞和效应性CTL。这些观察结果表明，GCT抗原的表达可能提供一种方法，用于识别肠道黏膜组织中最近受到刺激的pCTL或效应性CTL。
