D'Andrea A, Fasman G, Wong G, Lodish H
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Int J Cell Cloning. 1990 Jan;8 Suppl 1:173-80. doi: 10.1002/stem.5530080716.
We recently isolated a cDNA clone encoding the murine erythropoietin (Epo) receptor from a PXM expression library made from uninduced murine erythroleukemia cells. The clone was identified by screening COS cell transfectants for internalization of radiolabeled recombinant human Epo. As inferred from the cDNA sequence, the murine Epo receptor (Epo-R) is a 507 amino acid polypeptide with a single membrane-spanning domain. Extensive homology was found between the Epo-R and the interleukin 2 receptor beta chain, both at the level of amino acid sequence and at the level of regional hydrophobicity.
我们最近从由未诱导的小鼠红白血病细胞构建的PXM表达文库中分离出一个编码小鼠促红细胞生成素（Epo）受体的cDNA克隆。通过筛选COS细胞转染子中放射性标记的重组人Epo的内化来鉴定该克隆。从cDNA序列推断，小鼠Epo受体（Epo-R）是一种具有单个跨膜结构域的507个氨基酸的多肽。在氨基酸序列水平和区域疏水性水平上，Epo-R与白细胞介素2受体β链之间都发现了广泛同源性。
