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小鼠促红细胞生成素受体的表达克隆

Expression cloning of the murine erythropoietin receptor.

作者信息

D'Andrea A D, Lodish H F, Wong G G

机构信息

Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142.

出版信息

Cell. 1989 Apr 21;57(2):277-85. doi: 10.1016/0092-8674(89)90965-3.

DOI:10.1016/0092-8674(89)90965-3
PMID:2539263
Abstract

Two independent cDNA clones encoding the erythropoietin receptor (EPO-R) were isolated from a pXM expression library made from uninduced murine erythroleukemia (MEL) cells. The clones were identified by screening COS cell transfectants for binding and uptake of radioiodinated recombinant human erythropoietin. As inferred from the cDNA sequence, the murine erythropoietin receptor is a 507 amino acid polypeptide with a single membrane-spanning domain. It shows no similarities to known proteins or nucleic acid sequences in the data bases. Although the MEL cell EPO-R has a single affinity with a dissociation constant of approximately 240 pM, the EPO-R cDNA, expressed in COS cells, generates both a high-affinity (30 pM) and a low-affinity (210 pM) receptor.

摘要

从由未诱导的小鼠红白血病(MEL)细胞构建的pXM表达文库中分离出两个独立的编码促红细胞生成素受体(EPO-R)的cDNA克隆。通过筛选COS细胞转染子对放射性碘化重组人促红细胞生成素的结合和摄取来鉴定这些克隆。从cDNA序列推断,小鼠促红细胞生成素受体是一种含有单个跨膜结构域的507个氨基酸的多肽。它与数据库中已知的蛋白质或核酸序列没有相似性。尽管MEL细胞EPO-R具有单一亲和力,解离常数约为240 pM,但在COS细胞中表达的EPO-R cDNA产生了高亲和力(30 pM)和低亲和力(210 pM)两种受体。

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Expression cloning of the murine erythropoietin receptor.小鼠促红细胞生成素受体的表达克隆
Cell. 1989 Apr 21;57(2):277-85. doi: 10.1016/0092-8674(89)90965-3.
2
Erythropoietin receptor: cloning strategy and structural features.促红细胞生成素受体:克隆策略与结构特征
Int J Cell Cloning. 1990 Jan;8 Suppl 1:173-80. doi: 10.1002/stem.5530080716.
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Human erythropoietin receptor: cloning, expression, and biologic characterization.人促红细胞生成素受体:克隆、表达及生物学特性研究
Blood. 1990 Jul 1;76(1):31-5.
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Unregulated expression of the erythropoietin receptor gene caused by insertion of spleen focus-forming virus long terminal repeat in a murine erythroleukemia cell line.脾脏病灶形成病毒长末端重复序列插入小鼠红白血病细胞系导致促红细胞生成素受体基因的无调控表达。
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Characterization of murine erythropoietin receptor genes.小鼠促红细胞生成素受体基因的特征分析
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Inhibition of receptor binding and neutralization of bioactivity by anti-erythropoietin monoclonal antibodies.
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The cytoplasmic region of the erythropoietin receptor contains nonoverlapping positive and negative growth-regulatory domains.促红细胞生成素受体的胞质区域包含不重叠的正向和负向生长调节结构域。
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Specific binding of erythropoietin to its receptor on responsive mouse erythroleukemia cells.促红细胞生成素与其在反应性小鼠红白血病细胞上的受体的特异性结合。
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Induction of the receptor for erythropoietin in murine erythroleukemia cells after dimethyl sulfoxide treatment.二甲基亚砜处理后小鼠红白血病细胞中促红细胞生成素受体的诱导。
Cancer Res. 1988 Apr 1;48(7):1818-22.

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