Site-directed insertion mutagenesis with cloned fragments in Escherichia coli by P1 phage transduction.

A cloned gene with an insertion, which was made by introducing cat, was ligated to the cloning site of the phage lambda gt11. P1 phage grown on cells lysogenized with the recombinant lambda phage could transduce the mutant gene into the original site on the Escherichia coli chromosome.