通过在recD菌株中进行基因置换构建大肠杆菌K-12 ada缺失株，揭示了另一种修复烷基化DNA的甲基转移酶。
Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.
作者信息
Shevell D E, Abou-Zamzam A M, Demple B, Walker G C
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
J Bacteriol. 1988 Jul;170(7):3294-6. doi: 10.1128/jb.170.7.3294-3296.1988.
Abstract
We constructed an ada deletion by gene replacement in a recD1014 strain of Escherichia coli. Characterization of a delta ada-25 recD+ strain revealed the presence of a second DNA methyltransferase activity in E. coli K-12 which transfers a methyl group from methylated DNA to a protein with a molecular weight of 18,000 to 20,000.
摘要
我们通过基因置换在大肠杆菌的recD1014菌株中构建了一个ada缺失突变体。对Δada - 25 recD⁺菌株的特性分析表明，大肠杆菌K - 12中存在第二种DNA甲基转移酶活性，该酶可将甲基从甲基化的DNA转移至分子量为18,000至20,000的蛋白质上。
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