Benistant C, Rey C, Fonlupt P
Unité INSERM 205, Laboratoire de Chimie Biologique, INSA, Villeurbanne, France.
Neurosci Lett. 1990 Mar 2;110(1-2):137-42. doi: 10.1016/0304-3940(90)90801-f.
Incubation of membrane preparations from rat brain with S-adenosyl-L-methionine resulted in methylation of the lipid fraction. Neither [3H]diazepam nor [3H]muscimol (a gamma-aminobutyric acid (GABA) agonist) binding was affected by this incubation. In contrast, when [3H]diazepam binding was stimulated by GABA, the methylation caused both a decrease of the minimal GABA concentration needed to produce its effect (10(-9) M instead of 10(-7) M) and an enhancement (from 36 to 66% over basal) of the GABA-stimulated [3H]diazepam binding.
用S-腺苷-L-甲硫氨酸孵育大鼠脑的膜制剂会导致脂质部分甲基化。这种孵育对[3H]地西泮和[3H]蝇蕈醇（一种γ-氨基丁酸（GABA）激动剂）的结合均无影响。相反，当GABA刺激[3H]地西泮结合时，甲基化既降低了产生其效应所需的最低GABA浓度（从10(-7) M降至10(-9) M），又增强了（比基础值提高36%至66%）GABA刺激的[3H]地西泮结合。
