Ngou J, Segondy M, Graafland H
Laboratoire de Développement et de Production, Centre Régional de Transfusion Sanguine, Montpellier, France.
Res Virol. 1990 Jan-Feb;141(1):69-80. doi: 10.1016/0923-2516(90)90057-p.
The immunoblotting technique was used to analyse polypeptides of purified Epstein-Barr virus reacting with antibodies present in sera from clinically healthy individuals, from patients with infectious mononucleosis (IM) or AIDS, and from renal transplant recipients. Polypeptides with molecular sizes in the range of 40-290 kDa were detected. The 47- and 160-kDa nucleocapsid polypeptides, as well as the 72-, 74-, 140-, 220- and 290-kDa membrane polypeptides were the major viral proteins detected in the sera. Sera from clinically healthy individuals contained antibodies directed against all EBV membrane and nucleocapsid antigens. Sera from renal transplant recipients, from patients with IM and from patients with AIDS failed to react with certain nucleocapsid and membrane antigens; in particular, sera from AIDS patients and renal transplant recipients did not react with the 220-kDa polypeptide, one of the major membrane antigens, while sera from subjects with IM and from healthy individuals did. A high proportion of sera from patients with IM (38% vs 5% of clinically healthy individuals and 0-5% of the AIDS patients and renal transplant recipients) reacted with a 42-kDa polypeptide, suggesting its possible role in acute EBV infection.
采用免疫印迹技术分析纯化的爱泼斯坦-巴尔病毒的多肽，这些多肽与临床健康个体、传染性单核细胞增多症（IM）患者或艾滋病患者以及肾移植受者血清中的抗体发生反应。检测到分子量在40 - 290 kDa范围内的多肽。47 kDa和160 kDa的核衣壳多肽，以及72 kDa、74 kDa、140 kDa、220 kDa和290 kDa的膜多肽是血清中检测到的主要病毒蛋白。临床健康个体的血清含有针对所有EBV膜和核衣壳抗原的抗体。肾移植受者、IM患者和艾滋病患者的血清未能与某些核衣壳和膜抗原发生反应；特别是，艾滋病患者和肾移植受者的血清不与主要膜抗原之一的220 kDa多肽发生反应，而IM患者和健康个体的血清则会发生反应。IM患者中很大一部分血清（38%，而临床健康个体为5%，艾滋病患者和肾移植受者为0 - 5%）与一种42 kDa的多肽发生反应，提示其在急性EBV感染中可能发挥的作用。
