Mizuno Y, Ohba Y, Fujita H, Kanesaka Y, Tamura T, Shiokawa H
Section of Biochemistry, Hokkaido University, Sapporo, Japan.
Arch Biochem Biophys. 1990 May 1;278(2):437-43. doi: 10.1016/0003-9861(90)90282-4.
Distributions of acylphosphatase isozymes among organs of several animal species were investigated. Organ extracts of pig and chicken were treated with isozyme-specific antibodies, subjected to electrophoresis on a polyacrylamide gel, then the gel was stained for acylphosphatase activity. Both animals showed three activity bands; one band was named common type isozyme because of its wide distribution in testis, muscle, brain, heart, spleen, kidney, liver, and erythrocyte, and the other two bands were named muscle type isozymes because of their localization in skeletal muscle. This classification was supported by selective and quantitative reactions of the isozymes to the isozyme-specific antibodies. Because the two bands of the muscle type have the same amino acid sequence and differ only in modifications on an -SH group, it is suggested that pig and chicken have only the two major types of acylphosphatase. This conclusion was supported by similar experiments on dog, human, rabbit, and pigeon.
研究了几种动物物种各器官中酰基磷酸酶同工酶的分布情况。猪和鸡的器官提取物用同工酶特异性抗体处理，在聚丙烯酰胺凝胶上进行电泳，然后对凝胶进行酰基磷酸酶活性染色。两种动物均显示出三条活性带；其中一条带因其在睾丸、肌肉、脑、心脏、脾脏、肾脏、肝脏和红细胞中广泛分布而被命名为普通型同工酶，另外两条带因其定位于骨骼肌而被命名为肌肉型同工酶。同工酶与同工酶特异性抗体的选择性和定量反应支持了这种分类。由于肌肉型的两条带具有相同的氨基酸序列，仅在-SH基团的修饰上有所不同，因此表明猪和鸡只有两种主要类型的酰基磷酸酶。对狗、人、兔子和鸽子进行的类似实验支持了这一结论。
