Ohba Y, Mizuno Y, Shiokawa H
Section of Biochemistry, Hokkaido University.
J Biochem. 1989 Feb;105(2):173-7. doi: 10.1093/oxfordjournals.jbchem.a122635.
Activities of the two isozymes, Ch1 and Ch2, of chicken muscle acylphosphatase were measured in breast muscles, leg muscles, and livers of developing chicks from day 11 in ovo to day 15 of free life. Measurement was performed using rabbit antibodies which could selectively precipitate Ch1 or Ch2. The activity contents of both Ch1 and Ch2 in muscles were low before hatching but rapidly increased after hatching. Ch1 showed a more marked increase than Ch2. In liver, on the other hand, the activity contents of Ch1 and Ch2 remained low throughout the period of pre- and post-hatching.
在孵化11天的鸡胚到出生后15天的雏鸡中，测量了鸡胸肌、腿肌和肝脏中鸡肌肉酰基磷酸酶的两种同工酶Ch1和Ch2的活性。使用能够选择性沉淀Ch1或Ch2的兔抗体进行测量。孵化前肌肉中Ch1和Ch2的活性含量较低，但孵化后迅速增加。Ch1的增加比Ch2更为显著。另一方面，在肝脏中，Ch1和Ch2的活性含量在孵化前后整个时期都保持较低水平。
